55520-89-3Relevant articles and documents
Partition coefficients of ketones, phenols, aliphatic and aromatic acids, and esters in n-hexane/nitromethane
Kotowska, Urszula,Isidorov, Valery A.
scheme or table, p. 813 - 824 (2012/03/27)
Liquid-liquid partition is used in sample preparation and in countercurrent and liquid-liquid chromatographic separations. Partition coefficients are widely used in toxicology, environmental, and analytical chemistry. The K hn determination procedure for the n-hexane/ nitromethane system was optimized and partition coefficients for 99 ketones, esters and trimethylsilyl derivatives of phenols, aliphatic and aromatic acids were determined. For 130 compounds, Khn values were predicted using mathematical relationships between Khn and other physicochemical and structural parameters. Versita Sp. z o.o.
Monitoring of the fermentation media of citric acid by the trimethylsilyl derivatives of the organic acids formed
Ghassempour, Alireza,Nojavan, Saeed,Talebpour, Zahra,Amiri, Ali Asghar,Najafi, Nahid Mashkouri
, p. 6384 - 6388 (2007/10/03)
In this approach, a derivatization method is described for monitoring of organic acids in fermentation media without any separation step. The aqueous phase of fermentation media was evaporated and heated in a silylation reagent to form trimethylsilyl (TMS) derivatives. The silylated compounds are analyzed by 29Si nuclear magnetic resonance (29Si NMR) and gas chromatography-mass spectrometry (GC-MS). 29Si NMR can qualitatively monitor the components produced in the Krebs cycle. Quantification of these compounds is investigated by using selected ion monitoring mode of mass spectrometry. In this mode, mass to charge (m/z) values of their [M - 15] + ions, which are 465, 275, 247, 221, 335, 251, and 313 of TMS derivatives of citric, α-ketoglutaric, succinic, fumaric, L-malic, oxaloacetic, and palmitic (as an internal standard), acids, respectively, are used. The limit of detection and the linear working range for derivatized citric acid were found to be 0.1 mg L-1 and 10-3 × 104 mg L-1. The relative standard deviation of the method for five replicates was 2.1%. The average recovery efficiency for citric acid added to culture media was ~97.2%. Quantitative results of GC-MS are compared with those obtained by an ultraviolet-visible method.