75269-16-8Relevant articles and documents
NUCLEOSIDES AND NUCLEOTIDES. PART 27. SOLID PHASE SYNTHESIS OF A TRIDECANUCLEOSIDE DODECAPHOSPHATE CONTAINING THE UNNATURAL BASE 4-AMINO-2(1H)-PYRIDINONE AND OF AN OCTANUCLEOSIDE HEPTAPHOSPHATE FOR THE DETERMINATION OF THE DNA-POLYMERASE SPECIFICITY
Charczuk, Roland,Tamm, Cristoph
, p. 717 - 725 (2007/10/02)
The tridecanucleoside dodecaphosphate d-(T-T-Z-C-G-T-C-A-A-A-A-T-C) incorporating the modified nucleoside 4-amino-1-(2'-deoxy-β-ribofuranosyl)-2(1H)-pyridone (Zd, 1) and the octanucleoside heptaphosphate d(G-A-T-T-T-T-G-A) were synthesized by the solid-phase method using the triester method.The carrier material was a copolymer consisting of styrene with 1 percent divinylbenzene.
N,N-BISPHOSPHORODIAMIDIC CHLORIDE: A NOVEL COUPLING REAGENT IN THE SYNTHESIS OF OLIGODEOXYRIBONUCLEOTIDES.
Katti, S. B.,Agarwal, K. L.
, p. 2547 - 2550 (2007/10/02)
Use of a novel coupling reagent, N,N-bisphosphorodiamidic chloride (BOPDC) in the synthesis of oligodeoxyribonucleotides is described.This reagent allows the synthesis of phosphotriesters in high yields (70-80 percent) without detectable side reactions.Synthesis of a hexanucleotide, d(A-A-C-C-C-G) is presented as an example.
Simplified Liquid-Phase Preparation of Four Decadeoxyribonucleotides and their Preliminary Spectroscopic Characterization
Denny, William A.,Leupin, Werner,Kearns, David R.
, p. 2372 - 2393 (2007/10/02)
Four self-complementary decadeoxyribonucleotides, dApTpApTpApTpApTpApT,dApTpApTpCpGpApTpApT, dApTpApTpGpCpApTpApT and dApApApApApTpTpTpTpT, were chemically synthesized by the phosphotriester procedure.Efforts were concentrated on keeping the procedure as simple as possible, concomitant with obtaining high-purity products at each step of the process.The decamers were elaborated from the 3'-end, starting with a 3'-O-benzoylated monomer according to the scheme: monomer + monomer -> dimer + dimer -> tetramer + dimer -> hexamer + tetramer -> decamer.Putity of intermediates and of the fully blocked decamers were monitored by chromatography and by 300-MHz 1H-NMR. spectroscopy.The deblocked decadeoxyribonucleotides were characterized by their UV., CD., and 1H-NMR. spectra.