• Name

  2' 7'-DICHLOROFLUORESCIN DIACETATE FOR&

• EINECS 685-843-8
• CAS No. 4091-99-0
• Density 1.463g/cm³
• Solubility
• Melting Point 209-210 °C(lit.)
• Formula C₂₄H₁₆ Cl₂ O₇
• Boiling Point 593.5 °C at 760 mmHg
• Molecular Weight 487.293
• Flash Point 312.7 °C
• Transport Information
• Appearance
• Safety

  Safety Statements	22-24/25
  WGK Germany	3
  F	10-21

• Risk Codes 36/37/38
• Molecular Structure
• Hazard Symbols Xi
• Synonyms Benzoicacid, o-(2,7-dichloro-3,6-dihydroxyxanthen-9-yl)-, diacetate (7CI,8CI);2',7'-Dichlorodihydrofluorescein diacetate;2',7'-Dichlorofluorescin diacetate;H2DCFDA;2-(2,7-dichloro-3,6-diacetyloxy-9h-xanthen-9-yl)-benzoic acid;
• PSA 99.13000
• LogP 5.82810

Synthetic route

2044-85-1

2',7'-dichlorodihydrofluorescein diacetate

4091-99-0

2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid

Conditions

Conditions	Yield
With palladium 10% on activated carbon; hydrogen In ethyl acetate at 20℃; under 760.051 Torr; for 18h;

4091-99-0

2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid

107-19-7

propargyl alcohol

Conditions

Conditions	Yield
With dmap; dicyclohexyl-carbodiimide In dichloromethane at 20℃; for 1h;	61%

4091-99-0

2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid

2,7-dichlorofluorescein

2,7-dichlorofluorescein

Conditions

Conditions	Yield
With dihydrogen peroxide; horseradish peroxidase In water
Multi-step reaction with 2 steps 1: sodium hydroxide / ethanol / 0.5 h / 20 °C 2: 3-(N-morpholino)propanesulfonic acid; cysteamine; air / CuSO4; 1,10-phenanthroline / 25 °C / pH 7 View Scheme
With Ac-IHIHIQI-CONH2; water; copper(II) ion pH=8; Kinetics; Reagent/catalyst;
Multi-step reaction with 2 steps 1: sodium hydroxide / ethanol 2: C35H29CuN3O4 / ethanol; aq. phosphate buffer / pH 7.4 View Scheme

4091-99-0

2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid

106070-31-9

2-(2,7-dichloro-3,6-dihydroxy-9H-xanthen-9-yl)benzoic acid

Conditions

Conditions	Yield
With sodium hydroxide In ethanol at 20℃; for 0.5h;
With sodium hydroxide for 0.5h; cooling;
With sodium hydroxide In ethanol at 20℃; for 0.5h; Darkness;

4091-99-0

2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid

76-54-0

2',7'-dichlorofluorescein

Conditions

Conditions	Yield
With hemin; d(ATTGTGCGTCATCCCTTACGTCAGTGGAG), screened from Cauliflower mosaic virus (CaMV) 35S promoter; d(CTCCACTGACGTAATGGGTAGGG); d(GGGTTGGGCGGATGACGCACAAT); potassium chloride; ammonium acetate In water at 20℃; for 0.166667h; pH=7; aq. buffer;
With water; oxygen Irradiation;

4091-99-0

2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid

C29H22Cl2O7

Conditions

Conditions	Yield
Multi-step reaction with 2 steps 1: dicyclohexyl-carbodiimide; dmap / dichloromethane / 18 h / 20 °C / Inert atmosphere 2: ammonium bicarbonate / tetrahydrofuran; methanol; water / 18 h / 20 °C / Inert atmosphere; Darkness View Scheme

4091-99-0

2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid

C35H30Cl2O7

Conditions

Conditions	Yield
Multi-step reaction with 3 steps 1: dicyclohexyl-carbodiimide; dmap / dichloromethane / 18 h / 20 °C / Inert atmosphere 2: ammonium bicarbonate / tetrahydrofuran; methanol; water / 18 h / 20 °C / Inert atmosphere; Darkness 3: tetra(n-butyl)ammonium hydrogensulfate; potassium carbonate / dichloromethane; water / 18 h / 20 °C / Inert atmosphere; Darkness View Scheme

4091-99-0

2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid

C26H18Cl2O5

Conditions

Conditions

Yield

Multi-step reaction with 4 steps 1: dicyclohexyl-carbodiimide; dmap / dichloromethane / 18 h / 20 °C / Inert atmosphere 2: ammonium bicarbonate / tetrahydrofuran; methanol; water / 18 h / 20 °C / Inert atmosphere; Darkness 3: tetra(n-butyl)ammonium hydrogensulfate; potassium carbonate / dichloromethane; water / 18 h / 20 °C / Inert atmosphere; Darkness 4: 2,3-dicyano-5,6-dichloro-p-benzoquinone / dichloromethane; water / 18 h / 20 °C / Inert atmosphere; Darkness View Scheme

7314-44-5

2,4-dimethoxylbenzyl alcohol

4091-99-0

2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid

C33H26Cl2O9

Conditions

Conditions	Yield
With dmap; dicyclohexyl-carbodiimide In dichloromethane at 20℃; for 18h; Inert atmosphere;	149.4 mg

4091-99-0

2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid

C50H45Cl3N6O12S

Conditions

Conditions	Yield
Multi-step reaction with 2 steps 1: dicyclohexyl-carbodiimide; dmap / dichloromethane / 1 h / 20 °C 2: copper(II) sulfate; tris[(1-benzyl-1H-1,2,3-triazol-4yl)methyl]amine; sodium L-ascorbate / tetrahydrofuran; water; ethanol / 2 h / 20 °C View Scheme

4091-99-0

2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid

C46H41Cl3N6O10S

Conditions

Conditions	Yield
Multi-step reaction with 3 steps 1: dicyclohexyl-carbodiimide; dmap / dichloromethane / 1 h / 20 °C 2: copper(II) sulfate; tris[(1-benzyl-1H-1,2,3-triazol-4yl)methyl]amine; sodium L-ascorbate / tetrahydrofuran; water; ethanol / 2 h / 20 °C 3: sodium hydroxide / methanol / 0.5 h / 37 °C View Scheme

2,7-Dichlorodihydrofluorescein diacetate Specification