Recombinant trypsin inhibitor

Details:

Recombinant Aprotinin

Catalog Number: RTI16

CAS: 9087-70-1

Synonyms: Trypsin Inhibitor

Source: Recombinant aprotinin is a genetically engineered protein expressed in E.coli

Storage temperature: Liquid,it should be stored at -20℃ or below;Powder,it should be stored at 2-8℃

Protein Sequence:The amino acid sequence of recombinant aprotinin is identical to bovine lung derived aprotinin

 

1.DESCRIPTION

Aprotinin is a competitive serine protease inhibitor that inhibits trypsin, chymotrypsin, kallikrein and plasmin. Aprotinin forms stable complexes with and blocks the active sites of enzymes. Binding is reversible with most aprotinin-protease complexes and dissociating at pH <3.0. Effective concentration is equimolar with protease. YaxinBio recombinant aprotinin is a genetically engineered protein expressed in E.coli and purified by multi-column.The amino acid sequence of recombinant trypsin is identical to bovine lung derived aprotinin,with equivalent properties compared to native aprotinin and can replace native trypsin for using in a variety of biotechnological processes.

 

2.MAIN FEATURES

Source	Recombinant E. coli
Appearance	Clear, colorless to yellowish liquid
Protein Concentrate	≥5.0
Specific Activity (EPU /mg protein)	≥3.0
Absorbance (277nm)	≤0.8(3 EPU/ml in water)
Purity (HPLC)	≥95%

UNIT DEFINITION:One trypsin inhibitor unit (EPU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmol of N-benzoyl-L-arginineethyl ether(BAEE) per sec at pH 7.6 at 25 °C.

A conversion factor for Aprotinin is: 1 EPU= 1 USP Aprotinin Unit

 

3.RECOMMEND USAGE

Effective concentration is equimolar with protease. The recommend binding is pH＞6.0 and it will dissociate at pH <3.0.

 

4.STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Recombinant aprotinin liquid should be stored under -20℃ in sealed container. It is stable within 6 months at 2-8℃. It is no activity loss after 6 times repeated freezing and thawing.

Stability of transport: The product is stable by blue ice insulation transport.

 

5.PRODUCT ADVANTAGE

Animal origin free: Recombinant aprotinin is no exogenous virus contamination, and any animal origin material is not used in the production process.

Stable quality: Mass production can ensure stable and continuous batch production. It is no difference between the batch and the product quality is stable.

High purity: Higher specific activity. Host protein residues is less than the limits of biological products.

Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.

Complete quality documents: We can provide relevant regular support files in according to customers requirement.

 

6.PRODUCT USAGE

Aprotinin is a competitive serine protease inhibitor that inhibits trypsin, chymotrypsin, kallikrein and plasmin. Aprotinin forms stable complexes with and blocks the active sites of enzymes. Recombinant aprotinin is with equivalent properties compared to native aprotinin and can replace native trypsin for using in a variety of biotechnological processes,such as inhibition of serine protease activity in recombinant protein production,cell culture and so on.