SYBR Green I for Real Time PCR, 10000x
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SYBR Green I for Real Time PCR, 10000x
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SYBR Green I is a sensitive dsDNA binding dye, which can be used for routine DNA detection in agarose and polyacrylamide gels.
Unlike Ethidium, SYBR Green I is highly selective towards double stranded DNA, much less harmful, and offer better sensitivity.
A comparison between Ethidium Bromide and SYBR Green I
| Feature | Ethidium Bromide | SYBR Green I |
|---|---|---|
| Fluorescence | Red (615 nm) | Green (524 nm) |
| Excitation maximum | 302 nm | 454 nm |
| Excitation light source | UV only | Blue light or UV |
| Sensitivity |
2 ng / band(dsDNA) 100 ng / band (RNA) |
0.08 ng / band (dsDNA) 1-2 ng / band (oligonucleotides) |
| Health hazard | High | Low |
General properties
| Appearance: | orange solution |
| Quality control: | NMR 1H (95%) and 13C, TLC, functional testing |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
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