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Introduction: Protein A is a cell wall protein of Staphylococcus aureus and contains five functional domains that can bind to the Fc fragment o…
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Introduction:
Protein A is a cell wall protein of Staphylococcus aureus and contains five functional domains that can bind to the Fc fragment of IgG. As an affinity ligand, protein A is conjugated to Sepharose and its functional domains are available for binding IgG. One molecule of conjugated protein A can bind at least two molecules of IgG.
Column characteristics
Some typical binding and elution conditions with protein A Sepharose
| Species | Antibody Class | Protein A binding | pH value of binding buffer | pH value of elution buffer |
| Human | IgG1 | ++ | 6.0~7.0 |
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