Application characteristics of 3-cyclohexylamine propanesulfonic acid CAPS

Application characteristics of 3-cyclohexylamine propanesulfonic acid CAPS

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1 Kilogram	FOB Price: USD 1.0000

1 Kilogram

FOB Price: USD 1.0000

Min.Order :1 Kilogram
Purity: ≥99%
Payment Terms : L/C,D/A,D/P,T/T,Other

Keywords

3-cyclohexylamine propanesulfonic acid CAPS CAPS Buffer

Quick Details

Appearance:White crystal powder
Application:At present, the PVD transmembrane of protein has not used Tris-gly, SDS, 20% methanol buffer, because there is glycine in the buffer, so it is difficult to distinguish whether glycine is brought in by
PackAge:500g/bottle, 25kg/barrel (can be packed according to customer's requirements)
ProductionCapacity:500|Kilogram|Month
Storage:Room temperature storage
Transportation:Export for many years, qualified for shipping and air transportation

Superiority:

1. Factory production, more preferential prices, regular products are available in stock;

2. DeSheng is a manufacturer of a series of biological buffer products, including Tris, Tris-HCL, Bicine, Caps, Mops, Taps, EPPS, HEPES, PEP, PIPES, MOPSO and so on. To provide you with more comprehensive services;

3. Desheng has been in the industry for 14 years. Our products are self-developed and manufactured. Biobuffer has high purity and guaranteed product quality.

4. Desheng began to do foreign trade in 2010. We have our own right to export.

Details:

3-cyclohexylamine propane sulfonic acid CAPS (CAS1135-40-6) has a molecular weight of 221.31. It is usually used as a biological buffer in related research experiments for several macromolecular substances such as nucleic acids and proteins. It is similar to Tris, MOPS, Bicine, etc. but also has a The difference is different, so what are its characteristics in application

Cyclohexylamine propane sulfonic acid has a pKa of 10.4 under standard conditions (standard atmospheric pressure 25 degrees Celsius). The appearance is white powder and fine crystals. The density is slightly higher than that of water. It is soluble in water but not very soluble. 100g of water can dissolve 9g of CAPS at room temperature, and its solubility in hot water will increase significantly. Therefore, in production, CAPS is purified by dissolving CAPS in hot water, and then adding ethanol for cooling and crystallization.

Characteristics of cyclohexylamine propanesulfonic acid in biochemical applications

From the dissociation constant value of cyclohexylamine propanesulfonic acid, we can know that CAPS is weakly basic. Although the propanesulfonic acid group in the molecule is weakly acidic, the cyclohexylamine group is basic to make the whole molecule appear weak. Weakly alkaline. Therefore, CAPS buffers are usually used in biological experiments with a pH in the range of 9.4-11.4 (alkalescence).

And buffers like Tris, Bicine, TAPS, the applicable pH range is usually close to the neutral pH 7, so CAPS is used as a buffer when separating basic drugs by high performance liquid chromatography (HPLC). Moreover, CAPS is also suitable for the reaction system of enzymes with a higher pH optimum, such as supporting the activity of alkaline phosphatase.

Cyclohexylamine propane sulfonic acid CAPS can reduce the yield of non-specific products of nucleic acid hybridization. CAPS, cholamidopropyl dimethyl ammonium propane sulfonate (CHAPS), cyclohexylamine hydroxy propane sulfonic acid are usually used for nucleic acid extraction. CAPSO and CHES are used together to prepare nucleic acid hybridization buffers, which can improve the specificity of nucleic acid hybridization, maintain the yield of target hybridization products, and play an important role in specific pathogen detection and gene sequence analysis.

CAPS is suitable for the separation and purification experiments of high molecular weight proteins (molecular weight greater than 20KD). In the protein PVDF transfer experiment, using CAPS instead of the Tris-glycine+20% methanol buffer system can reduce the amount of methanol, and it can also eliminate the interference caused by the glycine brought by the buffer during subsequent protein sequencing.

The above are the characteristics of CAPS in the application of cyclohexylamine propanesulfonic acid. It is also based on the feedback from Desheng customers. There are also some characteristics of CAPS applications in DNA and RNA-related experiments and the application of biochemical kits. We need everyone Summarize in practice.

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