Application and precautions of CHAPSO buffer

Details:

The chemical full name of biological buffer CHAPSO is 3-[(3-cholaminepropyl) dimethylamino]-2-hydroxy-1-propanesulfonic acid inner salt. Because the name is too long and inconvenient to remember, it is often abbreviated. appeared in the public eye. In addition to the two names mentioned above, it can also be found through its "ID number", which is CAS number 82473-24-3.
CHAPSO should be called zwitterionic surfactant accurately. It is a non-denatured zwitterionic bile salt derivative developed on the basis of CHAPS. Compared with CHAPS, it has more advantages. It can be used as a non-denaturing detergent in protein purification experiments, and has the characteristics of good water solubility, good pH stability and high purity.
Application of CHAPSO Buffer
1. As a detergent for dissolving macromolecular organisms such as proteins;
2. It is used to study the dissolution process of proteoliposomes;
3. As a lysate in the opioid receptor research experiment, the opioid receptor can be reversibly bound;
4. Orienting macromolecules in a new bicelle system;
5. Used in other experiments as a zwitterionic detergent.
Precautions for the use of CHAPSO buffer
About storage: For long-term storage, it must be sealed and protected from light, because CHAPSO powder is easy to absorb moisture, strictly sealed, and the product is not easy to deteriorate;
About use: When configuring and using, dissolve in strict accordance with the proportion, otherwise it will affect subsequent use;
Solution storage: The prepared solution, after high temperature sterilization, can be stored for a short time below 4°C, and it is best to use it immediately;
Safety Tips: It is harmful to contact or inhalation. Take safety precautions when using it. If the product comes into contact with the skin, rinse it with water as soon as possible or seek medical attention.
Hubei Xindesheng Material Technology Co., Ltd. is a manufacturer of CHAPSO buffers. It has been committed to the development and production of biological buffers for more than ten years. If you need to buy, please come to consult and understand!