The performance and advantages of the biological buffer Bicine

Details:

Bicine buffer, namely dihydroxyethylglycine, CAS number: 150-25-4, is a very versatile biological buffer. It has the same excellent buffering performance as Tris, and is gradually replaced in some demanding biochemical tests. Traditional phosphate buffer.

Bicine is an amino acid derivative, slightly acidic, with a pH buffer range of 7.6-9.0, soluble in water and commonly used organic solvents such as DMF, DMSO, DMAc, etc. When the powdered bicine is formulated into a solution, sodium hydroxide is usually added to adjust the pH value to meet the pH environmental requirements required by the experiment or detection. If the experimental environment needs to eliminate the interference of sodium ions, you can use potassium hydroxide instead of sodium hydroxide, then measure the pH with a pH meter, and adjust the mixing ratio of the Bicine solution and the lye.

Similar to the working principle of other biological buffers, Bicine is amphiphilic. When acid and alkali are added to the reaction system, or the pH of the reaction environment changes as the reaction progresses, it can act as a buffer and maintain the reaction system. The pH value is intuitively important for reactions involving enzymes or proteins and accounting in biochemical testing. The stability and functionality of most organic biomolecules are very sensitive to pH.

The molecular structure of Bicne is similar to that of glycine. The hydrogen on the amino nitrogen atom is replaced by two hydroxyethyl groups, which reduces the hydrolysis ability of the amino terminal and enhances its stability. It also has the properties of amino acids and aminoethanol and can be used as a single anion.
(Bic-) forms a metal complex. The buffer system configured with Bicine and sodium hydroxide will not participate in the chemical reaction of the reaction system, nor will it interfere with the experimental reaction, and is resistant to chemical and enzymatic hydrolysis.

Desheng is a manufacturer specializing in the production and development of biochemical testing and in vitro diagnostic raw materials. It has rich experience in blood pretreatment, blood pretreatment and biochemical kits. The current mature product series include blood collection tube additives, color reagents, and chemiluminescence. Reagents and biological buffers including more than a dozen Good's reagents such as Bcine.