The application of Bicine in in vitro diagnosis and other aspects

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Bicine (N,N-Dihydroxyethylglycine, CAS: 150-25-4) is a white powder, which is widely used as an organic compound as a buffer. Bicine is a zwitterionic biological buffer, usually used as a buffer in biological and biochemical research. It is one of Good’s buffers, with a pKa of 8.35 at 20°C. It is prepared by reacting glycine with ethylene oxide and then hydrolyzing the resulting lactone. Bicine can be used for pollutants in amine systems for gas desulfurization. It is formed by amine degradation in the presence of O 2, SO 2, H 2 S or thiosulfate.

As Good's Buffer, Bicine is usually used in protein crystallization solutions. Bicine is a very versatile buffer for enzyme reaction buffers and electrophoresis buffers. The working concentration is from 3-100mM. Used in biological buffers, especially for low-temperature biochemical research; used to prepare stable substrate solutions for the determination of serum guaninase; spacer arms in the fractionation of plasma proteins by isotachophoresis using cross-linked dextran.

Bicine has been used as a buffer in chromatography and polymerase chain reaction. Bicine is an amphoteric amino acid buffer and is active in the pH range of 7.6-9.0 (pKa of 8.3 at 25°C). It is the recommended buffer for low-temperature biochemical reactions. And it has the advantages of not participating in and not interfering with the biochemical reaction process, and being resistant to chemical and enzymatic hydrolysis, so it is often used as a buffer in chromatography and polymerase chain reaction.

The mobile phase buffer and eluent of Bicine in cation exchange chromatography, and the use of Bicine in thin-layer ion exchange for protein separation chromatography methods have been confirmed. Bicine has been used for peptide and protein crystallization. The quaternary transition state analog creatine kinase uses Bicine in the reaction buffer. A protein SDS-PAGE multiphase buffer system combined with Bicine peptides has been widely used in in vitro diagnostic clinical trials.

Used as a chelating agent for soil remediation. Because Bicine molecule contains two -OH and one -COOH, it has good chelating properties and can chelate heavy metal ions such as copper, cadmium, and lead ions, but cannot chelate calcium and magnesium ions. Therefore, Bicine is often used as a chelating agent to repair soil contaminated by heavy metals. Bicine|150-25-4| has a good elution ability for heavy metals under neutral and acidic conditions, and can efficiently remove Cu, Cd, and Pb ions in contaminated soil. Bicine|150-25-4| is The eluent of the active component can not only wash and repair heavy metal contaminated soil, but also avoid the loss of plant nutrient elements calcium and magnesium in the soil, which is safe and environmentally friendly, and will not cause secondary pollution to the environment.

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