Advantages of N,N-bis(hydroxyethyl)glycine Bicine in various biological buffers

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Now the commonly used biological buffers on the market: Tris, Tris-HCL, Bicine, Caps, Mops, Taps, EPPS, HEPES, PEP, PIPES, MOPSO, etc. Why did we choose Bicine First, let's understand what Bicine is What are its special advantages

The chemical name of Bicine is N,N-bis(hydroxyethyl)glycine (CAS:150-25-4) and the English name is N,N-Bis(2-Hydroxyethyl)Glycine, which is a white crystalline powder. It is soluble in water, but insoluble in organic solvents such as acetone, DMF, DMSO, DMAc, etc. N,N-Dihydroxyethylglycine is a pH buffering agent suitable for biochemistry and medical research, with a pH buffer range of 7.6-9.0 and a pKa value of 8.3 at 25°C. This property makes it often used as a buffer for capillary electrophoresis Solution. Its molecular structure is similar to glycine.

BICINE is a very versatile buffer. It is not only used to prepare a stable substrate solution for guaninase determination, but also used as an electrophoresis buffer with a working concentration of 3-100ml. The electrophoresis buffer is an important component of the nucleic acid and protein gel electrophoresis system, a conductor in the electrophoresis field, and a necessary condition for maintaining a constant pH value of the electrophoresis system.

Buffer solution refers to a mixed solution composed of weak acid and its salt, weak base and its salt, which can offset and reduce the influence of external strengthening acid or strong base on the pH of the solution to a certain extent, so as to keep the pH value of the solution relatively stable. In the biochemical research work, it is often necessary to use a buffer solution to maintain the pH of the experimental system.

The change of pH value of the solution system of research work often directly affects the effectiveness of research work. If the pH value of the "extracted enzyme" experimental system changes or changes significantly, the enzyme activity will decrease or even be completely lost. Therefore, the preparation of buffer solutions is an indispensable key step. Buffer solution is an important concept in inorganic chemistry and analytical chemistry. Buffer solution refers to a solution that can maintain the relative stability of pH. The pH value does not change significantly due to dilution or the addition of a small amount of acid or alkali within a certain range. The buffer solution has different pH values ??and buffering capacity according to the amount of conjugated acid-base pairs and their substances.

Desheng Technology will continue to develop and research advanced biochemical reagents around the needs of the medical laboratory field to meet the needs of the global medical laboratory technology development and strive for the cause of human health. All kinds of ready-to-use solutions customized by our company are welcome to consult, and the PH can be customized. Consultation hotline: 17321456337 (same number on WeChat).