Several issues worthy of attention to 4-hydroxyethylpiperazine ethanesulfonic acid (HEPES)

Details:

The chemical name of the biological buffer HEPES product is: 4-hydroxyethylpiperazine ethanesulfonic acid, which is a white crystalline powder. It is a weak acid and a zwitterionic buffer in organic chemical industry. It can be widely used in clinical laboratory diagnostic reagents, life science research, pharmacy and biotechnology and many other fields. Next, let's take a look at several issues worthy of attention about HEPES.

What role does HEPES play in the cell culture process
Answer: HEPES is a weak acid. The Chinese name is 4-hydroxyethylpiperazine ethanesulfonic acid. Its main function is to prevent rapid changes in the pH of the medium. Under open culture conditions, when observing cells, the culture medium is separated from the 5% CO2 environment, CO2 gas quickly escapes, and the pH rises rapidly. If HIEPES is added, the pH can be maintained at about 7.0 at this time. Generally, HEPES should be added during cloning culture.

What are the applications of HEPES in the kit
Answer: HEPES can prepare lactate dehydrogenase detection kits. This detection kit adopts a dual-reagent mode to effectively avoid the interference of non-specific reactions, while minimizing the interference from the turbidity of the sample itself, ensuring the stability and reliability of the measurement results, with good stability, high accuracy, and linear testing range Wide, good repeatability, strong anti-interference and other advantages.

What are the effects of HEPES used in skin care products
Answer: HEPES is harmless to the human body and is safe and stable. Its pH-maintaining properties can help skin care products maintain the activity of microbial fermented extracts and prolong the effect; HEPES can promote the skin's absorption of various components in skin care products and increase the skin's absorption rate of cosmetics ; HEPES also has the effects of softening cutin, enhancing cell metabolism, and synergistic sun protection.

Characteristics of 4-hydroxyethylpiperazine ethanesulfonic acid (HEPES)
Answer: The decomposition ability of HEPES can increase with the increase of temperature and decrease with the decrease of temperature. However, compared with other buffers, its decomposition constant does not change much with temperature. This makes HEPES buffers effective at low temperatures. A buffer that better maintains the structure and function of the enzyme. HEPES powder is resistant to high temperatures and has a melting point of up to 200°C, so it will not be degraded by autoclaving. However, it should be noted that when HEPES powder is placed in a dry room, it cannot be exposed to direct sunlight for a long time.

Desheng Technology is a professional enterprise engaged in the research, production and sales of scientific research and biochemical reagents. The company adopts advanced production technology and equipment, and a humanized office environment. It has gathered a group of energetic and pragmatic technical personnel and a strict matrix management team. The HEPES currently provided by Desheng is a powder reagent, and it can be used if required. Formulated into a ready-to-use buffer solution of a specific concentration.