HEPES buffer-a fighter in cell culture

Details:

HEPES solution is a weak acid, the Chinese name is hydroxyethylpiperazine ethanesulfonic acid, which is a zwitterionic buffer in organic chemical industry. The decomposition ability of this substance can increase with the increase of temperature, and decrease with the decrease of temperature. However, compared with other buffers, its decomposition constant does not change much with temperature, which makes HEPES buffers more effective at low temperatures. A buffer that keeps the structure and function of the enzyme well.

HEPES buffer can control a constant pH range for a long time, has no toxic effect on cells, and is often used in cell culture. In order to improve the culture environment of BHK-21 cells, maintain the stability of the foot-and-mouth disease virus 146S antigen, and increase the content of intact virus particles in the vaccine, some experts have conducted studies on the culture buffer system. In the study, a comparative test of adding HEPES on the stability of virus antigens was carried out to evaluate its protective effect.

According to the experimental results, the virus liquid cultured with the biological buffer HEPES has a higher virulence than the virus liquid without biological buffer. At 37°C, the TCID50 (half of the tissue culture infectious dose) of the virus fluid without biological buffer has a larger change than that of the virus fluid containing biological buffer. However, it should be noted that when HEPES is exposed to light, it will Hydrogen peroxide is produced, which is toxic to cultured cells or other biologically active objects. Therefore, the operation of HEPES as a buffer should be carried out under dark conditions as much as possible.

Therefore, it can be seen that the biological buffer can effectively improve the buffering capacity of the virus culture solution, provide a suitable environment for the virus, and promote the stability of the virus particle. If you need to buy HEPES buffer, please find Desheng Technology, a biochemical reagent company integrating scientific research, production and sales. Factory direct sales can save you a lot of purchase costs.