12 uses of HEPES Buffer you didn't know

Details:

Hepes is a zwitterionic biological buffer, commonly used in biological and biochemical research. It is one of the earliest Good buffers described by Norman E. Good in 1966. Although it is a very popular buffer in laboratories around the world, what is Hepes actually suitable for The following can better understand the characteristics of Hepes and its applicability to different biotechnology applications (cell culture, chromatography, etc.).

Basic Information:
CAS Number: 7365-45-9
Molecular weight: 238.3
Molecular formula: C 8 H 18 N 2 O 4 S
Useful pH range: 6.8-8.2
pKa (25°C): 7.45-7.65
Maximum wavelength (λmax)λ: 260 nm Amax: 0.06; λ: 280 nm Amax: 0.04

What can Hepes do
Research on most metal ions
 It can be a good substitute for Tris and phosphate in the research of metal ions
Used for environmental, analytical and biological research
As the binding buffer and eluent in cation exchange chromatography
As a grinding buffer in plant research
As the electrophoresis buffer in electrophoresis
As a buffer for electroporation
Buffer mammalian cell culture
As a buffer for in vitro fertilization and embryo culture
For Bradford or bicinchoninic acid (BCA) analysis
For research on cartilaginous amphibians, because it is non-toxic to this algae
It has been introduced into the extraction buffer to prevent the protein in the red blood cells from

 
Before choosing Hepes for research, what issues should you consider
uIt affects the membrane potential of neuronal cells
uIt will interfere with the determination of Lowry nucleic acid protein
uIt is not suitable for redox research
uIt is toxic to small crustaceans (water fleas), such as Daphnia magna and Daphnia pulex
uIt will interfere with the phenol oxidation of peroxidase
uIt will affect the self-oxidation rate of iron
uIt is not recommended to use Folin reagent for protein determination

Useful tips about Hepes:
Easily soluble in water
It has negligible metal ion bond
It can inactivate the popular RNase inhibitor DEPC

In the specific use of hepes, cosmetics are used as pH adjustment, which is conducive to the formation and stability of gold nanostars. It is also present in the prevailing virus preservation solutions today, which can be used for a wide range of purposes.