Preparation of different types of buffers for hydroxyethylpiperazine ethanesulfonic acid HEPES

Details:

HEPES, 4-hydroxyethylpiperazine ethanesulfonic acid, is a commonly used biological buffer, usually used in cell preservation solutions, DNA/RNA extraction kits, and PCR diagnostic kits. According to different needs, HEPES can be configured into a variety of different buffer systems.

HEPES buffer has many advantages, good buffering performance, and non-toxic to cells. It is a hydrogen ion buffer that can control the system in a constant pH range for a long time. Its pH buffer range is 6.8-8.2, and the final concentration is 10-50mmol/L. Generally, the buffering capacity can be achieved when the culture medium contains 20mmol/L HEPES.

Hydroxyethylpiperazine ethanesulfonic acid HEPES configures different buffers
According to the specific requirements of different experiments, the composition and preparation method of HEPES buffer are also different. Usually HEPES is combined with sodium hydroxide NaOH or HEPES+ salt.
1. When preparing the stock solution of HEPES, first weigh HEPES and dissolve it in distilled water, and then adjust the pH with 0.5-1M NaOH. Note that the effective pH buffer range is 6.8-8.2, and then distilled water to a constant volume and store at 4°C.

2. When preparing HEPES buffer salt solution, you need to add sodium chloride, potassium chloride, disodium hydrogen phosphate, dextran, etc., also use NaOH to adjust the pH, and finally store it at a constant volume and low temperature.

3. Leagene HEPES buffer, a commonly used solution in cell-cell adhesion experiments, is mainly composed of HEPES, sodium chloride, potassium chloride, phosphate, glucose, etc., and contains Ca2+, Mg2+ ions, pH 7.4, Sterilized by filtration。 Bacteria treatment. It is often used to clean tissues or cells (especially embryonic cells) during long-term aggregation culture to protect the cadherin of cells without affecting the formation of cell aggregation.

4. HEPES-BSA solution, referred to as HA solution for short, does not contain calcium and magnesium ions. It is one of the components of cell culture fluid and contains some other salt ions. After filtration and sterilization, it is mostly suitable for tissue cell primary culture. Wash and cultivate.

In addition to the above-mentioned buffers, HEPES can also be configured into many buffer systems, such as cell culture medium, Hanks storage medium, and virus storage medium containing HEPES. Desheng is a manufacturer specializing in the production of HEPES. The raw material is reagent powder, and different buffers can be configured according to needs.