2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid hepes

Details:

2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid is a very important biochemical reagent in the field of biochemical industry. It is usually used as a buffer in biochemical experiments, such as cell culture fluid, viral nucleic acid preservation solution, and so on. High-quality and low-cost HEPES products are inseparable from their advanced production technology.

Principle of HEPES synthesis:
Use sodium isethionate and ethylpiperazine to compound under high temperature, certain pressure and the action of a catalyst. Then the product is adsorbed by anion and cation resin and dehydrated. After concentration and extraction, methanol is added to the concentrated solution for alcohol precipitation and crystallization. The crystals are refined, spin-dried with a centrifuge, and finally sent to an oven for drying. After this series of extraction, crystallization, coupling separation, etc., through multi-step chemical unit operation and refinement, HEPES products can be prepared.

Hydroxyethylpiperazine ethanesulfonic acid HEPES manufacturer
This HEPES production method is an internationally advanced production technology method. The production process is pollution-free, waste-free, environmentally friendly and clean. The produced HEPES products have a pure white color and good quality, which can be used as high-quality and high-tech content in biological research. Biochemical reagent raw materials and pharmaceutical intermediates are widely used in many fields such as clinical laboratory diagnostic reagents, life science research, pharmacy and biotechnology.

The HEPES finished products obtained from the production need to be tested before they can be packaged and stored. The product quality indicators are as follows: purity:> 99. 5%; Ca content: <10ppm; Fe content: <5ppm; Pb content: <5ppm; moisture content: <0 045; Useful pH range: 6. 8-8.2; Normal temperature dissociation constant pKa (25 ℃): 7.5; Appearance: pure white crystalline powder.

HEPES is a hydrogen ion amphoteric buffer. It contains sulfonic acid groups, which makes it hydrophilic, so it can be directly dissolved in water. The dissociation constant at room temperature is 7.5, which makes its aqueous solution very close to neutral, so it is suitable for insulation. Most biochemical experiments.

Hydroxyethylpiperazine ethanesulfonic acid is as widely used as Tris Tris, and has low cytotoxicity. It has better performance than Tris. It is considered as a universal buffer in biological experimental research, but HEPES's The price is relatively high. Both of these buffers are produced by Desheng, which can be selected according to the actual situation.