caps buffer recipe

Details:

【Name】3-[Tris(hydroxymethyl)methylamino]-1-propanesulfonic acid

【Abbreviation】TAPS

【CAS#】29915-38-6

【Molecular weight】243.28

【Molecular formula】C7H17NO6S

【Storage Conditions】Room temperature, light-proof and moisture-proof

【Molecular structure】

Configuration method of caps

Since caps buffer is mainly composed of caps and deionized water, pH value is adjusted with sodium hydroxide to avoid contact with air. Caps added deionized water to 900 ml, adjusted pH value to 11.0 with 2 mol / L NaOH, then fixed volume to 1 L, stored in 4 ml º C。 Generally, the buffer solution with high methanol concentration is good for the transfer of low molecular weight protein, while the buffer solution with low methanol concentration or even without methanol is good for the transfer of high molecular weight protein. The concentration range of methanol in caps buffer is 0 ~ 20%, so caps buffer is more suitable for the transfer of low molecular weight protein.

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