CAPS buffer Western Blot

Details:

Cap buffer, 3- (cyclohexamine) - 1-propionic acid, cas135-40-6, is often used in Western blotting experiments. For example, the electrophoresis buffer made by caps is used in PVDF membrane of protein.

At present, most PVDF membranes of proteins in most laboratories do not use Tris Gly, SDS and 20% methanol buffer. Because there is glycine in Tris Gly buffer, it is difficult to distinguish whether glycine is taken in or protein itself.

Cap

However, the use of caps buffer does not affect sequencing. Now the membrane transfer buffer used for sequencing is 10mm/1caps10% methanol, and the methanol concentration range in caps electric imprinting buffer is 0-20%. Generally, buffer with high methanol concentration has good transfer effect on low molecular weight protein, while buffer with low methanol concentration or even without methanol is conducive to the transfer of high molecular weight protein.

Of course, for the sequencing of proteins with low molecular weight, Tricine buffer can be used instead of Tris Gly, and the problem of glycine in buffer solution can be avoided. This suitable protein molecular weight is generally below 20kD, and for egg white matter molecules greater than 20kD, it is suitable for the buffer system of caps.

Before the operation of the film transfer, the electroplating groove is prepared. The electrophoresis instrument 0.45um (suitable for protein with molecular weight above 20KD) and 0.22um (suitable for the protein with molecular weight below 20KD) pore size PVDF film, cut slightly larger than the gel size, two 3MM filter paper, cut into gel size, methanol (100% purity), deionized water. Cap imprinted buffer solution (mother liquor) shall be dissolved with deionized water, pH value shall be adjusted to 11.0 with 2mol/l sodium hydroxide, and then fixed volume shall be kept to the concave level of scale line and stored in 4 º C。 Cap imprinted buffer (working fluid, 1% methanol 10% × Caps, mixed before use) 0.1% Coomassie brilliant blue G-250 40% methanol / 1% acetic acid, decolorizing liquid 50% methanol.

Besides being used in biological buffer, cyclohexanone propionic acid caps are often used in coatings or other industries. The caps of Desheng have not only analytical chemical reagents, but also industrial grade products, and other types of biological buffer raw materials, which can meet the needs of different customers.