What is the purpose of Tris buffer？

Details:

Title：Tris  CAS77-86-1

【Name】Trometamol

【Abbreviation】Tris

【CAS#】77-86-1

【Molecular weight】121.135

【Molecular formula】C4H11NO3

【Storage Conditions】Room temperature, light-proof and moisture-proof

【Appearance】White crystal powder

【Molecular structure】

Trimethylaminomethane is a zwitterionic buffer, which is often used in biology and scientific research experiments. Of course, there are many applications in medicine and industry. So what are the specific uses of different Tris buffers, how to use Tris buffers, and understand the characteristics of Tris and different biotechnology, such as electrophoresis or chromatography.

What is Tris recommended for

Commonly used in gel electrophoresis buffer (TAE or TBE).

Usually used for preparation of Laemmli buffer (used for denaturation and loading of protein samples in SDS-PAGE)

Anion exchange chromatography

Used to evaluate bacterial endotoxin

For capillary electrochromatography (CEC), due to its low ion mobility

Used for metal complexation

It can be used to correct acidosis

What problems should be considered before selecting Tris for research

It is not very effective below pH 7.5

It has a potential reactive primary amine and can be used as an inhibitor

It tends to permeate membrane, so it is not suitable for most cell culture work

Toxic to many mammalian cells

It is not suitable for the analysis of dioctylcornic acid (BCA)

It may interfere with the Bradford dye binding method for protein determination

Chelated calcium and other essential metals are well known

The pH value of the solution containing Tris is very temperature dependent, so it is very important to prepare Tris buffer at the service temperature

It may interact with bicarbonate / CO2 buffer system

According to the use of different industries, the considerations are different, and the requirements for the level of Tris will also be different, and the purity and some other indicators will also be relatively different, and these factors often need the in-depth communication between buyers and manufacturers to better provide.

Tips on Tris:

High solubility in organic solvents

It reacts with a variety of enzymes and inhibits several enzymes

It can inactivate the popular RNase inhibitor DEPC