What is Tris buffer

Details:

Title：Tris  CAS77-86-1

【Name】Trometamol

【Abbreviation】Tris

【CAS#】77-86-1

【Molecular weight】121.135

【Molecular formula】C4H11NO3

【Storage Conditions】Room temperature, light-proof and moisture-proof

【Appearance】White crystal powder

【Molecular structure】

Tris is known in Chinese as trimethylol aminomethane, aminobutanol, bradykinine, 2-amino-2 - (hydroxymethyl) - 1,3-propanediol. It is a white crystal or powder. It is soluble in ethanol and water, slightly soluble in ethyl acetate and benzene, insoluble in ether and carbon tetrachloride, corrosive to copper and aluminum, and irritating chemicals.

Tris has high buffer capacity, high solubility in water and is inert to many enzyme reactions, which makes Tris a very satisfactory buffer for many biochemical purposes. Generally used to stabilize the reaction system, pH has a strong buffer capacity between 7.5-9.0.

Advantages of Tris buffer:

1. Because Tris base is more basic, we can only use this kind of buffer system to prepare the buffer with a wide range of pH from acidic to alkaline;

2. It has little interference to biochemical process and does not precipitate with calcium, magnesium and heavy metal ions.

Disadvantages of Tris buffer:

1. The pH value of the buffer is greatly affected by the concentration of the solution. When the buffer is diluted ten times, the pH value changes more than 0.1;

2. The temperature effect is large, and the temperature change has a great influence on the pH value of the buffer. The pH value of the buffer at 4 ℃ is 8.4, and the pH value at 37 ℃ is 7.4. The tris HCl buffer prepared at room temperature can not be used for 0-4;

3. It is easy to absorb CO2 in the air, so the prepared buffer should be tightly sealed;

4. This buffer has a certain interference effect on some pH electrodes, so the electrode compatible with tris solution should be used.

Application of Tris buffer:

The pH value of the buffer system was stable; The Tris-HCL buffer system was used to stabilize pH value in the gel. It is widely used as a solvent for nucleic acids and proteins; The low ionic strength of Tris buffer can also be used for the formation of intermediate fibers; The "Te buffer" was prepared by adding EDTA into Tris hydrochloric acid buffer, which can be used for DNA stabilization and storage; The "Tae buffer" can be obtained by changing the acid solution adjusting pH value into acetic acid, and the "tbe buffer" can be obtained by changing it into boric acid. These two solutions were used in the experiment of nucleic acid electrophoresis.