What is the pH of Tris?

Details:

Tris: trimethylaminomethane, an organic compound, whose molecular formula is (hoch2) 3cnh2, Tris is usually

TAE and tbe buffers (for the dissolution of nucleic acids) used in biochemical experiments, and their amino groups can be condensation and reverse with aldehydes

Should.

Tris is weak base, and the pH of Tris base water solution is about 10.5. Hydrochloric acid is generally added to adjust the pH value to the required value,

The buffer solution with this pH value can be obtained. The effective buffer range of buffer is between pH7.0 and 9.2, but the temperature should be noted

The effect of TKA on Tris is 8.1 at 25 ℃.

Because Tris buffer is a weak alkaline solution, DNA will be depolymerized in such a solution, thus improving its solubility

Solvability. EDTA is often added to Tris hydrochloric acid buffer to make "Te buffer", which is used for DNA

Stability and storage of. If the pH adjusted acid solution is replaced with acetic acid, the "Tae buffer" is obtained

(tris/acetate/edta), and boric acid is replaced to obtain "tbe buffer". These two kinds of slowness

The flushing solution is usually used in the nucleic acid electrophoresis experiment.

Tris HCl, Tris EDTA preparation:

1、 1000ml of 1mtris HCl (pH7.4, 7.6, 8.0)

Preparation method:

1. weigh 121.1gtris and put it in 1000ml beaker.

2. add about 800ml of deionized water and stir and dissolve it fully.

3. add the pH value required for adjusting the concentration HCl amount in the following table.

PH concentration HCl

7.4 about 70ml

7.6 about 60ml

8.0 about 42ml

4. the solution shall be fixed to 1000ml.

5. after high temperature and high pressure sterilization, store at room temperature.

Note: the solution should be cooled to room temperature and then adjusted to the pH value, because the pH value of Tris solution varies greatly with the temperature,

The pH value of the solution decreased by about 0.03 units every 1 ℃ increase of temperature.

2、 1000ml of 1.5mtris HCl (ph8.8)

Preparation method:

1. weigh 181.7gtris in a 1000ml beaker.

2. add about 800ml of deionized water and stir and dissolve it fully.

3. adjust the pH value to 8.8 with concentrated HCl.

4. the solution shall be fixed to 1000ml.

5. after high temperature and high pressure sterilization, store at room temperature.

Note: the solution should be cooled to room temperature and then adjusted to the pH value, because the pH value of Tris solution varies greatly with the temperature,

The pH value of the solution decreased by about 0.03 units every 1 ℃ increase of temperature.

3、 Te is tris edtabuffer (10mmtris, 1mmedta, ph7.4ph7.6ph8.0).

ten × Tebuffer (pH7.4, 7.6, 8.0) 100mmtris HCl, 10mmedta preparation 1000ml

Preparation method:

1. take the following solution and put it in 1000ml beaker.

①1MTris-HClBuffer(pH7.4，7.6，8.0)100ml；

②500mMEDTA(pH8.0)20ml

2. add about 800ml of deionized water to the beaker and mix evenly.

3. after the solution is fixed to 1000ml, it is sterilized at high temperature and high pressure.

4. store at room temperature.

Because Tris buffer is widely used, in order to highlight the advantages of Desheng in biological buffer products, we

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