Purification of Tris and its application in Biochemistry

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Tris, also known as tromethamine, is widely used in organic synthesis, coatings, fireproof materials, biochemistry and medicine. Among them, the purity of Tris used in industry will be a little lower, which needs further purification in the biochemical field. In the biochemical field, Tris is mainly used as a biological buffer.

Purification of industrial Tris

When Tris is used in biological buffer, it needs analytical reagent. If it is industrial Tris, it can be purified to become analytical reagent. Purification steps: dissolve Tris in 2:3 methanol solution at 60 ℃, then add 1% activated carbon by mass of Tris, keep temperature at 50 ℃ for half an hour, and then filter while hot. The filtrate is concentrated and crystallized at 80 ℃ under reduced pressure. After separation and crystallization, it is rinsed with anhydrous alcohol. After drying, Tris reagent with high purity can be obtained.

Purification and biological application of Tris

Application of Tris in biological buffer

In biochemical experiments, Tris is the most common buffer after phosphate buffer. It is usually used together with acid to maintain the pH value of the reaction system. Tris hydrochloric acid buffer, Tris acetic acid buffer, and glycine, boric acid, malic acid can also be used to synthesize buffer system. Enzymes are involved in most of the tests of in vitro diagnosis, and appropriate pH is needed to play the catalytic role of enzymes. In the past, phosphate buffer was widely used, but in the system containing calcium, magnesium and other metal ions, phosphate would form precipitation, while Tris buffer would not.

Tris for protein purification

Tris is a good solvent for protein, and can maintain the appropriate pH value of protein, keep the spatial structure of protein unchanged, and prevent protein denaturation. Proteomics is to further understand the mechanism of life activities and the molecular mechanism of disease from the level of proteomics. In the research of proteomics, protein extraction is one of the most basic and key links, and Tris is a commonly used reagent in protein extraction.

In addition, Tris can also be used for electrophoresis experiments of nucleic acid DNA. The electrophoresis buffer in DNA agarose gel electrophoresis or polyacrylamide gel electrophoresis is Tris buffer system, such as TAE, TBE or even TPE. Desheng is a Tris manufacturer with mature Tris production and purification technology, which can provide industrial grade and analytical reagent.