What is the difference between Tris and Tris HCl?

Details:

What is the difference between Tris and Tris HCl In short, Tris is an alkaline buffer, and Tris HCl is an acid buffer. Remember that buffer is used to resist pH changes, even if there is no buffer, even a small amount of strong acid or strong alkali can significantly change the pH value of the environment. Therefore, the reason for selecting between Tris HCl or Tris also depends on the objectives achieved by the buffer to be used.

In this article, what are the differences between them studied by their application and when to choose one of them

1. Tris buffer: Tris is an organic compound, mainly used in molecular biology to make buffer or as basic buffer. What are the buffers that use Tris buffers frequently

CTAB DNA extraction buffer

Tae buffer

Tbe buffer

Te buffer

Tris buffer

Tris glycine buffer

In addition to the commonly used buffer, researchers also used changes in pH in Tris buffers or as buffers in more specialized procedures.

2. Tris HCl buffer: Tris HCl is a common buffer used by molecular biologists to regulate the pH value or stable pH value of the solution. Tris HCl is a Tris with HCl added, and what buffer is often used

CTAB DNA extraction buffer

Lemmli buffer for SDS PAGE

Tae buffer

Tbe buffer

Te buffer

Tris HCl buffer

For these buffers, it is up to the ongoing experiment to determine whether Tris HCl is to be used or Tris is used. Conjugated acid or alkaline components can be used when the pH value of the solution needs to be adjusted. For example, if there is a requirement for Tris HCl, Tris can be used to adjust pH.

In conclusion, if the pH of Tris buffer needs to be reduced, select Tris HCl. However, if the pH value of buffer solution needs to be increased, use Tris base. Above is some information provided by our company. If you have any purchase of biological buffer, please contact us.

Hubei xindesheng Material Co., Ltd. specializes in producing Tris, HEPES, mops and other buffer products. Up to now, the company has been established for decades, and has rich experience in the research and development of biological buffer. We can provide a large number of Tris and other types of biological buffer products, and we can customize and professional after-sales services for customers, and can provide professional product sample testing and packaging customization services for special customers. In any case, Desheng must be the best choice for you to buy biological buffers.