TOOS sodium salt

Details:

【Name】N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline

【Abbreviation】TOOS

【CAS#】82692- 93-1

【Molecular weight】295.33

【Molecular formula】C12H18NNaO4S

【Storage Conditions】Room temperature, light-proof and moisture-proof

【Transport conditions】Room temperature

【Oxidation reaction】

The chromogen substrate toos is a new Trinder's reagent. Its enzymatic reaction with hydrogen peroxide is highly sensitive and specific. It is usually used for the determination of the indicators that can produce hydrogen peroxide in biochemical detection. For example, the concentration of uric acid in human serum is determined by toos in the kit.

Chromogen substrate toos powder

The significance of uric acid determination

Uric acid is the product of purine metabolism in the body. Adenosine deaminase ADA catalyzes the deamination of adenine nucleoside to hypoxanthine nucleoside, which is finally metabolized into urea through a series of enzymatic reactions. The level of uric acid reflects the health status of the body. For example, the decrease of uric acid excretion or the hypermetabolism of purine may cause hyperuricemia and ventilation; Hypouricemia can be caused by too little or too much uric acid. Therefore, it is particularly important to maintain the normal level of uric acid in the body and the determination of uric acid level.

The uric acid was determined by toos

Under the catalysis of uricase, uric acid reacts with oxygen and water to produce allantoin and hydrogen peroxide. In the presence of peroxidase, the chromogen substrate toos and 4-aminoantipyrine are coupled and oxidized by the hydrogen peroxide generated in the previous step to produce red quinone imines. The darker the color, the more color products there are. The content can be measured by spectrophotometer, The absorbance is proportional to the content of uric acid.

In order to simplify the experimental operation and avoid the trouble of adding reagents repeatedly, the non reactive reagents can be divided into two groups. Reagent 1 is mainly composed of biological buffer, peroxidase and chromogen substrate toos; Reagents 2 were mainly uricase, 4-AAP and sodium azide. In the experiment, we only need to mix them in different proportions.

It should be noted that when measuring uric acid with toos, the amount of reagents and samples can be increased or decreased in proportion according to different instruments. If the uric acid content in the sample is too high (more than 1190umol / L), the sample can be diluted one by one with normal saline, and then the determination can be carried out, and the result can be multiplied by 2.

It is a common method to determine uric acid by chromogen substrate toos enzyme spectrophotometry. In addition, it can also be determined by chromogen tops or TMB enzyme-linked immunosorbent assay. Desheng is a manufacturer of IVD raw materials, providing a variety of chromogen substrates such as toos, tops and Maos.