isoluminol chemiluminescence

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The main derivatives of luminol are isoluminol, 4-aminohexyl-n-ethyl isoluminol, AHEI and Abei. Luminol can be oxidized by some oxidants under alkaline conditions, resulting in chemiluminescence reaction, and the maximum emission wavelength is 425nm.

In general, the chemiluminescence reaction of luminol with hydrogen peroxide is rather slow, but the reaction is very rapid in the presence of some catalysts. The most commonly used catalysts are metal ions. In a large concentration range, the concentration of metal ions is directly proportional to the luminous intensity, so the chemiluminescence analysis of some metal ions can be carried out. Using this reaction, the organic compounds containing metal ions can be analyzed, achieving high sensitivity. The second is to use the inhibition of organic compounds on luminol chemiluminescence reaction to determine the organic compounds with quenching effect on chemiluminescence reaction. Third, indirect determination of inorganic or organic compounds by coupling reaction. The fourth method is to label the derivatives of luminol such as isoluminol (Abei) on carboxylic acid and ammonia compounds, and then separate them by high performance liquid chromatography (HPLC) or liquid chromatography (LC), and then react with hydrogen peroxide potassium ferricyanide under alkaline conditions for chemiluminescence detection. Other separation methods can also be used, such as labeling the newly synthesized chemiluminescence reagent isoluminol isothiocyanate to yeast RNA, centrifugation and dialysis separation, and then chemiluminescence detection. In addition, N 2 (B 2 carboxypropionyl) isoluminol was used and its properties were studied.