The role of mops buffer

Details:

3-morpholine propanesulfonic acid (MOPs) is a kind of zwitterion buffer reagent commonly used in biochemistry and molecular biology. Mops itself belongs to good's buffer, which can be used not only as a buffer system for cell culture medium, but also as a common electrophoretic buffer and protein purification chromatographic buffer.

What experiments can mops be used in

1. Used as running buffer in electrophoresis and protein purification in chromatography; 2. Prepare a variety of agar medium for the culture of bacteria, yeast and mammalian cells. However, concentrations higher than 20 mm are not recommended for mammalian cells; 3. It can be used as lysis buffer for E. coli cells; 4. as an eluent in gel filtration chromatography. 5. It was used in Northern hybridization as a buffer for RNA isolation and membrane transfer; 6. It is suitable for the determination of biquinolinic acid (BCA); 7. For electron transfer and phosphorylation of chloroplast thin layer preparation.

8. It can be used in biochemical diagnostic kits, such as DNA / RNA extraction kit, PCR kit, creatinine determination kit, etc.

Mops transportation and preservation

Mops powder can be stored at room temperature for several years. However, after standing, the bulk powder may gather together to form a hard block, but its chemical properties will not be affected. Mops solution can be stored at 2-8 ℃ for at least 6 months with 0.2 ml μ High pressure sterilization is not recommended. If it is necessary to prepare the mops solution without nuclease, the water can be autoclaved first, and then the powder can be added to dissolve it

Characteristics of mops buffer

(1) It has good solubility in aqueous solution, and the aqueous solution is colorless and transparent;

(2) It is stable in chemistry, has no toxicity to biology and has no obvious permeation to biofilm;

(3) The dissociation constant (pKa value) of acid-base is stable, generally in the range of 6-8. The ability to release protons is stable, and it is less affected by ion concentration, solution composition and temperature;

(4) It is not the metabolite, activator or inhibitor of biochemical reaction series, nor the substrate of enzyme, that is, it does not react with a variety of enzymes and chemical reagents. When it is used in tissue culture, virus diagnosis and other tests, cells show high vitality.

(5) In fact, there is no characteristic absorption in the ultraviolet wave, and there is weak absorption when the wavelength is less than 230-240 nm, so it does not interfere with the usual photometric measurement.

(6) It has no or weak complexing ability to cations;

Zwitterionic buffer has many advantages, wide application and large market demand, and has been widely produced. Desheng technology can provide a variety of high-quality zwitterion buffer solutions, including HEPES, mops, caps and other superior products. The quality of HEPES and mops products has been affirmed and praised by customers in various industries.