Advantages of Tris buffer

Details:

Tris Chinese Name: tris (hydroxymethyl) aminomethane, the appearance of white crystalline powder, good water solubility, can also be dissolved with ethyl acetate, but the dissolution rate is relatively slow, not with ether, carbon tetrachloride, copper, aluminum corrosion. The pKa value of Tris at 25 ℃ is 8, and the buffer range is 7-9. The common Tris buffers are Tris HCl buffer, Tris phosphate buffer and various derivative buffers, such as TBS, Te, etc. Tris is a very important biological buffer, and its CAS number is 77-86-1.

In biochemical research, biological buffers are often used, and there are more than 20-30 varieties of biological buffers. Biological buffers are widely used in in vitro diagnostics and biochemical experiments. What are the advantages of Tris in biological buffers

1. Tris has high solubility in water and is inert to many enzymes.

2. Tris has a high buffer capacity, which is generally used to stabilize the pH of reaction system, and has a strong buffer capacity between ph7.5 and pH9.0.

3. Tris is very basic, so we can only use this kind of buffer system to configure a wide range of pH buffer from acidic to alkaline.

4. Tris has little interference to the biochemical process and will not precipitate with calcium, magnesium and heavy metal ions.

5. Tris buffer is widely used in biochemistry, cosmetics, in vitro diagnosis, molecular biology and coatings.

When using Tris buffer, we should also pay attention to that the pH value of Tris is greatly affected by temperature and concentration, so we should consider the use environment in the preparation process. Tris can easily absorb CO2 in the air, and the prepared buffer should be tightly sealed to avoid loss. In addition, Tris can react with various molecules to a certain extent, including RNase inhibitors, aldehydes, enzymes, DNA and common metals. It can also act with heavy metals, but it also plays an inhibitory role in some systems.

Tris interferes with some pH electrodes, so it is necessary to use the electrode which is compatible with tris solution. In addition, Tris is not suitable for the determination of biquinolinic acid (BCA), and Tris needs to wear gloves and goggles during the operation, because the inhalation, intake and skin absorption of Tris will have a certain impact on the human body.

Desheng biotechnology has been developing and producing biological buffers for many years, and has invested a lot of money in the R & D and production of Tris series products. At present, the company's products have been sold to more than 100 countries around the world, product quality is reliable, price concessions can be discussed, interested can call consultation, Desheng biotechnology welcome your call.