Effect of EDTA as anticoagulant in vitro

Details:

In some clinical tests in hospitals, whole blood or plasma examination is used. Venous blood is usually collected for examination. Different anticoagulants will be added according to different examination requirements. In the specific field of in vitro diagnosis, anticoagulants are usually added to the blood collection vessel to prevent blood coagulation and form blood clot, so as to maintain the fluidity of blood and facilitate blood detection. Ethylenediaminetetraacetic acid (EDTA) is a kind of polybasic acid, containing four carboxylic acid groups and two amine groups, with lone pair electrons, which can chelate calcium and other metal ions. Calcium is essential for various enzyme reactions in coagulation cascade. EDTA combines with calcium ion to form chelate. The removal of calcium can effectively prevent blood coagulation in the collecting tube.

Potassium EDTA powder

Ethylenediaminetetraacetic acid (EDTA) has been recommended as the first choice anticoagulant for whole blood cell analysis and detection, because it can well preserve the morphology and composition of cells. In recent decades, due to the complexity of blood detection, the potential application scope of EDTA anticoagulant has been significantly expanded, which can be used to stabilize various experimental blood cells, It can protect unstable molecules.

Main use of ethylenediaminetetraacetic acid (EDTA): it is suitable for many hematological tests, especially for platelet count. EDTA-K2 is most suitable for whole blood cell analysis and hematocrit determination. International Committee for hematology Standardization (ICSH) recommends that EDTA-K2 be used as anticoagulant for blood cell count.

Note: EDTA can inhibit platelet aggregation and is not suitable for coagulation test and platelet function test.

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