Application characteristics of Tris buffer

Details:

Tris buffer, or Tris buffer, is usually used with hydrochloric acid to adjust the pH value to prepare buffer. Some of them are adjusted with acetic acid, boric acid, glycine and other acids. Many other kinds of buffers, such as bicine, mops, HEPES, caps, etc., are adjusted with sodium hydroxide alkali, which is the unique feature of Tris buffer.

Application of Tris in biochemical experiment

Basic properties of trimethylol aminomethane

The pKa of Tris is 8.1 at room temperature (25 ℃); According to the buffer theory, the effective buffer range of Tris buffer is between pH 7.0 and 9.2. The pH value of Tris aqueous solution is about 10.5. Generally, hydrochloric acid is added to adjust the pH value to the required value to obtain the buffer solution with this pH value. The pKa of Tris is greatly affected by temperature, which will lead to the change of pH value. Therefore, attention should be paid to the effect of temperature.

Characteristics of Tris buffer

Trihydroxymethyl aminomethane itself is a weak base, and the aqueous solution is alkaline, so hydrochloric acid is used to adjust the buffer solution instead of sodium hydroxide. Tris molecular structure contains an amino group, which can react with aldehydes. Therefore, the use of Tris buffer in the system containing aldehydes may be affected.

Tris buffer is suitable for nucleic acid related experiments

Tris buffer is usually neutral and weakly alkaline. DNA nucleic acid molecules will be deprotonated in such a solution to improve the solubility. Therefore, Tris can be used as a good solvent for nucleic acid (and also suitable for protein). In nucleic acid related experiments, Tris is preferred as a buffer. In Tris hydrochloride buffer, EDTA is often added to make te buffer, which can inhibit nuclease, inhibit the degradation of nucleic acid and improve the stability of nucleic acid. Acetic acid or boric acid is often used instead of hydrochloric acid in electrophoresis buffer, such as tae buffer and tbe buffer used in nucleic acid electrophoresis experiment.

In addition to nucleic acid related experiments, Tris is often used in protein related experiments, such as protein growth experiments under different pH conditions, using Tris as buffer. Because Tris buffer does not contain metal ions and has low ionic strength, it can also be used for the formation of intermediate fibers of laminin. Desheng is a manufacturer of buffers, which can supply more than tons of Tris.