What is the difference between tbe and Tae?

Details:

TBE buffer and TAE buffer are commonly used gel electrophoresis buffer. Tbe and Tae buffer can also keep pH and ion concentration during electrophoresis because the change of pH value will affect the net charge of nucleic acid. Both tbe and Tae buffer contain EDTA to prevent nucleic acid degradation by nuclease.

Tris buffer

What is the difference between tae and tbe

The difference between tae and tbe buffer is the composition of chemical composition. The main component of tbe is boric acid, while Tae buffer contains acetic acid. When nucleic acids pass through agarose matrix, these weak acids provide appropriate ionic concentration. Tbe represents Tris borate EDTA. It consists of Tris base, boric acid and EDTA. This buffer is often used in agarose gel electrophoresis. In addition, it is commonly used to analyze the DNA products of PCR by agarose gel electrophoresis or polyacrylamide gel electrophoresis. Tae represents Tris acetate EDTA. The buffer contains Tris base, glacial acetic acid and EDTA. It is usually used as an electrophoretic buffer in gel electrophoresis to separate nucleic acids.

Tbe buffer is suitable for the following situations:

1. Tbe is suitable for smaller segments with higher resolution, less than 2 kb. The use of small fragments can provide a clearer band.

2. Tbe has higher buffering capability than TAE, so it is more suitable for long-term operation.

Tae buffer is suitable for the following situations:

1. Tae can better separate larger fragments larger than 3 KB.

2. TAE is more suitable for cloning because tbe contains borates, which are inhibitors of many enzymes and are not conducive to cloning.

3, TAE is more suitable for extracting DNA from agarose gel.