Uses of BICINE buffer

Details:

In biochemical research work, buffer solution is often used to maintain the pH value of the experimental system. The change of pH value of the solution system in research work often directly affects the effectiveness of our work. Bicine is an amphoteric amino acid buffer with pH buffer range of 7.6-9.0, which is recommended for low-temperature biochemical reaction. As a buffer, bicine does not interfere with the biochemical reaction process, Bicine is resistant to chemical action and enzymatic hydrolysis. What other applications does bicine have besides being used as a biological buffer

1、 Bicine is often used as a buffer solution for capillary electrophoresis

Bicine is similar to glycine in molecular structure. It has a substituted amino group, a carboxyl group and two hydroxyl groups as coordination groups. Therefore, bicine has the properties of amino acid and aminoethanol at the same time. It can form a metal complex with a single anion (BIC -), and is often used as a buffer solution for capillary electrophoresis. Electrophoretic buffer is an important component of the gel electrophoresis system of nucleic acid and protein. It is also a conductor in the electrophoresis field and a necessary condition for maintaining the constant pH value of the electrophoresis system.

2、 Mobile phase buffer and eluent of bicine in cation exchange chromatography

The application of bicine in thin layer ion exchange has been confirmed for protein resolution chromatography. Bicine has been used for peptide and protein crystallization. Bicine was used in the reaction buffer. A protein

The multiphase buffer system of SDS-PAGE combined with bicine peptide has been widely used in in vitro diagnostic clinical trials

In the middle.

3、 Bicine can be used as a chelating agent in soil remediation.

Bicine is a kind of water-soluble amino acid analogue, which is an environmental protection material. It contains two - oh and one - COOH in the molecule. It has good chelating performance, and can chelate Cu, CD, Pb and other heavy metal ions, but can not chelate calcium and magnesium ions. Therefore, bicine is often used as a chelating agent for the remediation of heavy metal contaminated soil. The leaching solution with bicine as the active component can not only leach and repair heavy metal contaminated soil, but also avoid the loss of calcium and magnesium in soil, which is safe and environmentally friendly and will not cause secondary pollution to the environment.

Desheng is a professional supplier of biological buffers, providing buffers such as bicine, caps, mops, Tris, MOPSO, HEPES and other in vitro diagnostic reagents. It has independent R & D team and professional advantages in synthesis. Its product quality and technology have been widely recognized and used by customers at home and abroad. The purity of bicine produced by Desheng technology is more than 99%, the chloride ion content is less than 0.01%, the water solubility is good, the process is stable, and the appearance of the product can be guaranteed to be pure white crystal powder.