Configuration and application of caps buffer

Details:

Caps, full name of 3-cyclohexylamino-propanesulfonic acid, is white powder crystal particles, which can dissolve with water, but the dissolution rate is slow. The CAS number is 1135-40-6, and the molecular formula is c9h19no3s. It belongs to biological buffer. It is mostly used in biochemical diagnostic kit, PCR diagnostic kit, DNA extraction kit and RNA extraction kit. But what applications are there besides these What are the applications of CPSA.

1. Caps is used as biological buffer: caps is mainly used in biochemical diagnostic kit, DNA / RNA extraction kit and PCR diagnostic kit, as buffer for enzyme chemistry and HPLC separation of alkaline drugs. Caps can also support alkaline phosphatase activity and inhibit the growth of Aeromonas at pH 10.5. Caps can also be dissolved in deionized water and adjusted to pH 11.0 for the purification of fibronectin.

Caps needs better purity when it is used as biological buffer. Generally, it needs to analyze the purity. When it is used in industry, the requirement for purity is relatively low, but it also needs different treatment for different applications.

2. Caps in industry: it is used for new coatings and new materials. Caps is also the raw material for welding materials, air conditioning equipment and lithium metal.

3. It is used as analytical reagent, heat exchange carrier and pharmaceutical industry. It is used for air conditioning, pyrotechnics, dry batteries, lithium metal, flux and desiccant.

4. For the coating industry, it is used as water-based isohydric ester curing agent. Waterborne isocyanate curing agent can coexist with resins containing active groups (hydroxyl, carboxyl, amino, epoxy, etc.) for a long time at room temperature.

The application of caps is about these. The following will introduce the general configuration method of caps

Since caps buffer is mainly composed of caps and deionized water, pH value is adjusted with sodium hydroxide to avoid contact with air. Caps added deionized water to 900 ml, adjusted pH to 11.0 with 2 mol / L NaOH, then fixed volume to 1 L, stored at 4 ° C. Generally, the buffer solution with high methanol concentration is good for the transfer of low molecular weight protein, while the buffer solution with low methanol concentration or even without methanol is good for the transfer of high molecular weight protein. The concentration range of methanol in caps buffer is 0 ~ 20%, so caps buffer is more suitable for the transfer of low molecular weight protein.

Hubei xindesheng Materials Co., Ltd. specializes in the production and development of biological buffer and blood collection additive, enzyme preparation, chemiluminescent reagent, luminescent substrate and other products. Desheng has been established for decades, has its own R & D team, and has researched and developed biological buffers for many years. Its products include Tris, HEPES, taps, mops, caps, bicine, EPPs, pipes and a series of biological buffers. At present, the products produced by Desheng have been sold to many countries all over the world, all of which have received high praise and re purchase, and have reached long-term cooperation with many enterprises. Interested in understanding can call consultation, Desheng welcome your call.