Function and configuration of electrophoretic buffer mops

Details:

In the electrophoresis experiment, buffer will be used to maintain a constant pH value of the electrophoresis stability system, because in the electrophoresis experiment, its positive electrode and negative electrode will have an electrolytic reaction, the positive electrode will have an oxidation reaction, and the negative electrode will have a reduction reaction. Long time electrophoresis can make the positive electrode acid and the negative electrode alkali. The buffer solution can keep the pH of the two poles of the solution unchanged. It can also make the solution have a certain conductivity, which is conducive to the migration of DNA molecules.

Many people have thought of biological buffer MOPS 3- morpholine propane sulfonic acid. It belongs to Good 's buffer and has good solubility in water. The buffer range is between 6.5-7.9. It can be used as an electrolyte system for two-dimensional gel electrophoresis with medium intensity electrophoretic (IEF). It can also be used in Northern hybridization as a buffer for RNA isolation and membrane transfer.

In electrophoretic buffer   Common 10 × The configuration method of mops buffer is as follows:

(1) Weigh 41.8 g mops and dissolve it in about 700 ml DEPC treated water;

(2) Adjust the pH value to 7.0 with 2 n NaOH;

(3) 20 ml of 1 m NaOAc and 20 ml of 0.5 m EDTA (pH 8.0) were added to the solution;

(4) Volume to 1 l with 0.45 ml μ The impurities were removed by filtration with m membrane and stored in dark at room temperature.

In addition to mops buffer, there are many biological buffers used in electrophoresis, which are different from each other and suitable for all kinds of electrophoresis. In the current market, there are many manufacturers of biological buffers, and the product specifications and quality are uneven. Desheng is a manufacturer specializing in the production of biological buffers. Now it produces a large number of biological buffers. It has been established for more than ten years. It has rich R & D and production experience and product knowledge, and can provide a lot of technical support and after-sales guarantee for customers. If necessary, please feel free to contact us!