Tris buffer has many uses in electrophoresis

Details:

High quality Tris buffer is an important part of electrophoresis. It can make the current pass through the sample while resisting the pH change of the whole solution. The choice of buffer depends on the isoelectric point of the analyzed sample. In DNA electrophoresis, the commonly used buffers are Tris acetate EDTA and Tris borate EDTA. SDS (sodium dodecyl sulfate) is usually used in protein electrophoresis. These buffers help to separate samples into readable gels. Other buffers can help improve results or expand readings at specific molecular weights, depending on the sample.

Some knowledge about electrophoretic buffer has been obtained through professional literature recently. I want to share and discuss it with you here.

The role of Tris- acetate -EDTA and Tris- borate -EDTA in agarose gel electrophoresis:

Tris is a strong base and boric acid is an acid. The combination of the two can maintain the pH in the neutral range of 8 to 8.5. Under this alkaline condition, DNA is protected and can be separated correctly. EDTA plays an important role in this combination. EDTA is a chelating agent. It chelates Mg2 + ions required for DNase as a cofactor. DNase is an enzyme that cuts DNA into small fragments. Therefore, the addition of EDTA can protect our DNA from enzymatic activity. In addition, the buffer will neutralize the charge of water molecules. Under the action of current, water molecules decompose into H + and oh -. H + ions can react with PO3 - of DNA. Therefore, the negative charge of the buffer neutralizes the charge in the water and protects the DNA.

Although Tris acetate EDTA and Tris borate EDTA play the same role in electrophoresis, they have their own advantages and disadvantages. Tris acetate EDT buffer could interfere with enzymatic reaction and protect DNA, while Tris borate EDTA buffer could not.

In conclusion, Tris acetate EDTA and Tris borate EDTA derived from Tris buffer are very important in electrophoresis, which is one of the reasons why Tris buffer is in short supply in today's market. At present, it is very difficult to find a manufacturer specializing in the production of Tris last time. Here I recommend our company, Hubei xindesheng Material Co., Ltd.

Hubei xindesheng Material Co., Ltd. specializes in producing Tris, HEPES, mops and other buffer products. Up to now, the company has been established for decades and has rich experience in the R & D and production of biological buffer. We can provide a large number of Tris and other types of biological buffer products, and our company can provide customers with product customization and professional after-sales service, as well as professional product sample testing service and packaging customization service for special customers. In any case, Desheng company must be your best choice to buy biological buffer.