Purification of Tris and its application in biochemical field

Details:

Trimethylolaminomethane Tris, also known as aminobutanol, is widely used in organic synthesis, coatings, fireproof materials, biochemistry and medicine. Among them, the purity of Tris used in industry will be slightly lower. It needs further purification in the biochemical field. In the biochemical field, it is mainly used as a biological buffer.

Industrial Tris purification method

When Tris is used as biological buffer, analytical pure reagent is required. If it is industrial Tris, it can be purified to become analytical pure reagent. Purification steps: dissolve Tris in 2:3 methanol solution at 60 ℃, then add 1% activated carbon by mass of Tris, keep it at 50 ℃ for half an hour, filter it while hot, concentrate and crystallize the filtrate under reduced pressure at 80 ℃, wash it with anhydrous alcohol after separation and crystallization, and dry it to obtain high-purity aminobutanol Tris reagent.

Purification and biological application of Tris

Tris for biological buffer

In biochemical experiments, Tris is the most widely used buffer after phosphate buffer. It is usually used together with acid to maintain the pH value of the reaction system. The common ones are Tris hydrochloric acid buffer, Tris acetic acid buffer, and can also synthesize buffer systems with glycine, boric acid, malic acid, etc. Enzymes are involved in most tests of in vitro diagnosis, and appropriate pH is needed to play the catalytic role of enzymes. In the past, phosphate buffer was widely used. However, in the system containing calcium, magnesium and other metal ions, phosphate will generate precipitation. At this time, Tris buffer will not generate precipitation.

Tris for protein purification

Tris is a good solvent for protein, and can maintain the appropriate pH value of protein, keep the spatial structure of protein unchanged and prevent protein denaturation. Proteomics is to further understand the mechanism of life activities and the molecular mechanism of disease from the proteomic level. In the research of proteomics, protein extraction is one of the most basic and key links, and Tris is a common reagent in protein extraction.

In addition, Tris can also be used for electrophoresis experiments of nucleic acid DNA. The electrophoresis buffer in DNA agarose gel electrophoresis or polyacrylamide gel electrophoresis is Tris buffer system, such as TAE, TBE or even TPE. Desheng is the manufacturer of Tris. It has mature Tris production and purification technology and can provide industrial grade and analytical pure reagents.