Sodium 3-[Ethyl(m-tolyl)amino]-2-hydroxy-1- propanesulfonate

Details:

The chromogen substrate TOOS is a new Trinder's reagent. Its enzymatic reaction with hydrogen peroxide has high sensitivity and specificity. It is usually used to determine the indexes to be measured that can produce hydrogen peroxide in biochemical detection. For example, the concentration of uric acid in human serum is determined by toos in the kit.

Chromogen substrate toos powder

Significance of uric acid determination:

Uric acid is the product of purine metabolism in vivo. Adenosine deaminase ADA catalyzes the deamination of adenine nucleoside to hypoxanthine nucleoside, which is finally metabolized into urea through a series of enzymatic reactions. The level of uric acid reflects the health status of the body, such as decreased uric acid excretion or hypermetabolism of purine, which may cause hyperuricemia and ventilation; Too little uric acid production or too much excretion will cause hypouricemia. Therefore, it is particularly important to maintain the normal level of uric acid and the determination of uric acid level.

Determination of uric acid by toos enzyme method:

Under the catalysis of uricase, uric acid reacts with oxygen and water to produce allantoin and hydrogen peroxide. In the presence of peroxidase, the chromogen substrate toos is coupled with 4-aminoantipyrine and oxidized by hydrogen peroxide generated in the previous step to produce red quinoimines. The darker the color, the more color products, and its content can be measured by spectrophotometer, The absorbance is directly proportional to the content of uric acid.

In order to simplify the experimental operation and avoid the trouble of adding reagents repeatedly, the unreacted reagents can be divided into two groups. Reagent 1 is mainly biological buffer, peroxidase and chromogen substrate toos; Reagent 2 is mainly uricase, 4-AAP and sodium azide. The experiment only needs to mix the two in different proportions.

It should be noted that when measuring uric acid with toos, the amount of reagent and sample can be increased or decreased in proportion according to different instruments. If the content of uric acid in the sample is too high (greater than 1190umol / L), the sample can be diluted one by one with normal saline, and then measured, and the result can be multiplied by 2.

Determination of uric acid with chromogen substrate toos enzyme spectrophotometry is a common method in the kit. In addition, it can also be determined with chromogen tops or TMB enzyme-linked immunosorbent assay. The principle is basically similar. Desheng is a manufacturer of IVD raw materials, providing a variety of chromogen substrates such as toos, tops and Maos.