What role does Tris buffer play in DNA extraction

Details:

Tris , fully known as trimethylolaminomethane, is a common biological buffer, which can be used in the whole DNA extraction process. In the extraction process from multiple sources, DNA extraction is very sensitive to pH. Tris is required to maintain a stable pH during cell lysis, removal of unwanted cell components and precipitation. In addition, Tris buffer plays a special role in cell lysis.

Cell lysis is the first step of DNA extraction. This can be done by a buffer containing Tris and EDTA (ethylenediamine tetraacetic acid). EDTA binds divalent cations such as calcium and magnesium. Because these ions help to maintain the integrity of the cell membrane, eliminating them with EDTA will destroy the stability of the cell membrane. Tris is the main buffer component; Its main function is to maintain the pH value of the buffer at a stable value (usually 8.0). In addition, Tris may interact with LPS (lipopolysaccharide) in the membrane, which further destroys the stability of the membrane.

Tris buffer protects DNA from pH changes

When cells divide, their DNA and contents overflow into the buffer. This cell inclusion and fragmented RNA and protein have a great impact on the pH value of the solution. Because DNA is sensitive to pH, it is important for Tris buffer to stabilize pH.

In the final stage of DNA extraction, DNA itself is extracted from the solution. At this time, DNA is soluble in buffer. In order to extract from the solution, DNA can be made insoluble by adding ethanol or isopropanol. After this operation, the DNA will appear as a white linear substance in the solution. Although DNA can be isolated from the remaining cellular components in this way, it is "unavailable" when DNA is insoluble. After separation, the alcohol is removed and the DNA must be put back into Tris buffer before use.

In conclusion, Tris buffer plays many roles in DNA extraction, and it shows that Tris buffer plays an important role. This is also the reason why Tris buffer has become a hot commodity in the market, which makes the procurement very difficult. Here we recommend our company, Hubei xindesheng Material Co., Ltd.

As a manufacturer specializing in the production of biological buffer, Hubei xindesheng Material Co., Ltd. provides a large number of high-quality Tris and many other types of biological buffer products. We can customize the product specifications and provide after-sales service according to customer needs, and we can provide professional testing services and packaging customization services to special customers. In any case, Desheng company must be your best choice to buy biological buffer.