lowest price Proteinase K powder good supplier 39450-01-6 High quality Proteinase K

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lowest price Proteinase K powder good supplier 39450-01-6 High quality Proteinase K

Proteinase K [EC 3.4.21.64] is a stable and highly reactive serine protease enzyme belonging to the subtilisin family. Proteinase K [EC 3.4.21.64] contains two disulfide bridges and one free Cys near the active site His. Proteinase K [EC 3.4.21.64] has an active-site catalytic triad (Asp39-His69-Ser224). Proteinase K [EC 3.4.21.64] hydrolyzes peptide amides. Proteinase K [EC 3.4.21.64] catalyzes the reaction of keratin hydrolysis, as well as other proteins with substilisin-like specificity. Originally sourced from the mold Tritirachium album Limber, Proteinase K [EC 3.4.21.64] is also formerly known as the previous designations EC 3.4.4.16 and EC 3.4.21.14.

Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins. It cleaves at the carboxylic acid side of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. This enzyme was designated proteinase K because of its ability to hydrolyze keratin. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9.

Proteinase K is typically used at a concentration of 50-100 μg/ml. It is active with or without the presence of SDS, urea, EDTA or various metal ions, but the activity of proteinase K can be increased by adding the denaturing agents and the structure of proteinase K can be stabilized by addition of Ca ²⁺. Proteinase K is inactivated by heating to 95°C for 10 minutes or using an inhibitor such as PMSF, AEBSF or DFP.

GoldBio's Proteinase K undergoes two sterile filtration steps during the separation and purification processes. The final product is further analyzed by plate culture to ensure the removal of all living organisms was 100% effective.