Advantages of bicine buffer

Details:

Bicine buffer, namely dihydroxyethyl glycine, CAS No. 150-25-4, has superior buffer performance like TR is. It is a widely used biological buffer and gradually replaces the traditional phosphate buffer in some biochemical tests with high requirements. The demand is also growing.

Bicine buffer, dihydroxyethyl glycine, CAS No. 150-25-4, is a widely used biological buffer. Like Tris, it has good buffer performance and gradually replaces the traditional phosphate buffer in some biochemical tests with high requirements

Bicine is an amino acid derivative with weak acidity, pH buffer range of 7.6-90, soluble in water and common organic solvents such as DMF, DMSO, DMAC, etc. When preparing powder bicine into solution, sodium hydroxide is usually added to adjust the pH value to meet the pH environmental requirements required for experiment or detection. If the interference of sodium ions needs to be eliminated in the experimental environment, potassium hydroxide can be used instead of sodium hydroxide, and then the pH can be measured by pH meter to adjust the mixing ratio of bicine solution and alkali solution.

Similar to the working principle of other biological buffers, bicine is amphoteric. When acid and alkali are added to the reaction system or the pH of the reaction environment changes with the progress of the reaction, it can play a buffer role. It is intuitive and important to maintain the p value of the reaction system. When there is enzyme or egg white and accounting involved in the biochemical detection, The stability and functionality of most organic biomolecules are very sensitive to pH

Bicne's molecular structure is similar to that of glycine. The hydrogen on the amino nitrogen atom is replaced by two hydroxyethyl groups, which reduces the hydrogen content at the amino end

It has the properties of amino acid and aminoethanol, and can form gold with single anion (BIC -)

It is a complex. The buffer system composed of bicine and sodium hydroxide will not participate in the chemical reaction of the reaction system, nor interfere with the experimental reaction, and can resist chemical and enzymatic hydrolysis.

Desheng is a manufacturer specializing in the production and R & D of biochemical testing and in vitro diagnostic raw materials. It has rich experience in blood pretreatment, blood pretreatment and biochemical kit. At present, the mature product series include blood collection additives, chromogenic reagents, bioluminescent reagents and biological buffers including more than ten kinds of goods reagents such as bcine. Professional manufacturers are trustworthy.