How does HEPES buffer work in cell culture?

Details:

HEPES  buffer is a biological buffer used in cell culture. In chemistry, buffer is a solution containing acid and alkali or salt. Its main purpose is to maintain the hydrogen ion concentration at a specific regional level to maintain the required optimal pH range. HEPES, like other buffers, can maintain the pH value unchanged. Specifically, the pH range of HEPES buffer belongs to the ideal condition for maintaining cell culture, so it is suitable for microbial and histological research.

HEPES buffer

Application scope of HEPES in cell culture:

Its pH value range is 2.5-3.5 for acidic solution and 6.8-8.2 for alkaline solution, that is, HEPES buffer can be prepared into an ideal pH value range in any cell culture according to the type of cultured cells, such as eukaryotic cells, prokaryotic cells, unicellular organisms or tissue cells.

How does HEPES work in cell culture

In many experimental studies, cultured cells have many uses, such as diagnosing some diseases, gene analysis, identifying microbial species, measuring the efficacy of antibiotics, growing alternative skin tissue for skin transplantation, experimenting with cancer cells and extracting biomolecules. The water in the culture medium, such as agar, reacts with carbon dioxide to produce carbonic acid, which will affect cell culture. Therefore, the main function of adding HEPES buffer to cell culture medium during cell culture is to maintain the pH value of culture medium and maintain a good culture environment when carbon dioxide accumulates due to cell respiration.

When using the medium containing HEPES buffer, pay attention not to be exposed to visible light for a long time. Visible light will lead to the accumulation of hydrogen peroxide in the solution, and hydrogen peroxide will lead to cell death. Therefore, in order to prevent the formation of hydrogen peroxide, we must pay attention to dark culture.

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