Preparation method of biological buffer HEPES powder

Details:

HEPES , whose Chinese name is 4-hydroxyethyl piperazine ethane sulfonic acid, is a biological buffer, which has a wide range of applications. Perhaps when it comes to biological buffer, we first think of its application in biochemical experiments. We think it is far from our daily life. In fact, it is not. If you carefully observe the commonly used skin care products and cosmetics, you will find that there is 4-hydroxyethyl piperazine ethane sulfonic acid.

HEPES buffer powder

Due to the special buffer range of HEPES, it has an irreplaceable position in the field of industry, cosmetics and biochemical experiments. Due to the large market demand, HEPES is also in short supply in the market. So how is it produced Xiaobian here summarizes a method for preparing HEPES. Let's have a look.

Preparation steps of HEPES powder:

1. The addition reaction of vinyl sulfonic acid or vinyl sulfonate with N-hydroxyethyl piperazine to prepare 4-hydroxyethyl piperazine ethane sulfonate;

2. 4-hydroxyethyl piperazine ethanesulfonate was converted into 4-hydroxyethyl piperazine ethanesulfonic acid and the salt of corresponding acidifier with acidifier to obtain acidified mother liquor;

3. The acidification mother liquor is crystallized, and the salt of the corresponding acidifier is removed to obtain the treated acidification mother liquor;

4. Soluble barium salt or calcium salt is added to the acidification mother liquor, the residual sulfate is removed, evaporated and concentrated to obtain the primary purified product of HEPES;

5. The primary purified product was washed with small molecular alcohol and dried to obtain high-purity HEPES powder.

This method can not only produce high-purity HEPES, but also has the advantages of low purification cost, simple preparation process, easy operation and no pollution. Desheng biochemical is a source manufacturer of biological buffers. Since its establishment in 2005, it has rich experience in the R & D and production of biological buffers. At present, there are more than ten kinds of biological buffers. If necessary, you are welcome to consult and buy them on the official website.