Function and preparation method of Tris buffer

Details:

Buffer solution is an aqueous solution containing weak acids and their conjugated bases. Due to the chemical properties of buffer solution, buffer solution can maintain its pH value (acidity) at an almost constant level even if the substance changes chemically. Buffer naturally exists in plasma, which keeps the pH value of blood between 7.35-7.45. In organic systems, natural buffers maintain pH at a consistent level. However, in the process of biological research, the pH value must be kept constant through buffer. There are many kinds of buffers, and Tris buffer is one of the commonly used biological buffers.

Tris powder

What is Tris buffer

Tris is the abbreviation of tris (hydroxymethyl) aminomethane, which is a compound commonly used in salt water. Because it is isotonic and non-toxic, PKA is 8.1 and pH value is between 7 and 9, Tris buffer solution is also commonly used in a series of chemical analysis and biological research, including DNA extraction. It is important to know that the pH value in Tris buffer solution will change with solution temperature.

How to prepare Tris buffer

The preparation of Tris buffer is very simple. Only the following materials are prepared

● tris (hydroxymethyl) aminomethane (Tris)  

● distilled deionized water

● hydrochloric acid

step

1. First determine the concentration (molar concentration) and volume of Tris buffer you want to make. For example, Tris buffer solutions for normal saline range from 10 to 100 mm. According to the experimental requirements, the required number of moles of Tris is calculated by multiplying the molar concentration of the buffer by the volume of the buffer being prepared. (moles of Tris = mol / L x L)

2. Next, determine how many grams this is by multiplying the number of moles by the molecular weight of tris (121.14 g / mol). The number of grams of Tris = (mole) x (121.14 g / mol)

3. Dissolve Tris in distilled deionized water to 1 / 3 to 1 / 2 of the required final volume.

4. Mix in HCl (e.g. 1m HCl) until the pH meter provides the required pH for your Tris buffer solution.

5. Dilute the buffer with water to reach the required final solution amount.