Application of dipotassium EDTA-2K

Details:

Dipotassium ethylenediaminetetraacetate (EDTA-2K for short) is a white crystalline powder, insoluble in water and slightly soluble in alcohol. The pH value of its aqueous solution is about 5.3 and the melting point is 272 ° C. Ethylenediamine tetra acetic acid two potassium is widely used. It can be used for complexing metal ions and separating metals. It is also used in detergents, liquid soap, shampoo, agricultural chemical spray and antidote. It is commonly used in blood anticoagulants.

Dipotassium ethylenediamine tetraacetate (EDTA-2K)

Dipotassium EDTA can be used for whole blood cell counting. Whole blood cell analysis is widely used in clinical detection. Platelet count has become an important basis for the diagnosis and treatment of clinical thrombosis and hemorrhagic diseases, and it is also one of the important parameters for preoperative detection of surgical patients. The International Committee on blood standards recommends the use of potassium ethylenediamine tetraacetate (EDTA-K2) anticoagulant for whole blood cell count.

In addition, the detection of total bilirubin (TBIL), direct bilirubin (DBIL), total protein (TP), albumin (ALB), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and γ－ The results of 8 liver function indexes such as glutamyltransferase (GGT) and alkaline phosphatase (ALP) were compared. Methods the above 8 biochemical tests were performed on EDTA-K2 anticoagulant plasma and serum on automatic biochemical analyzer, and the results were compared and evaluated. The results showed that there was no significant difference in TBIL, TP, ALB, ALT and AST between EDTA-K2 anticoagulant plasma and serum. The ALP of EDTA-K2 anticoagulant plasma was significantly lower than that of serum, the difference was statistically significant, GGT was slightly lower than that of serum, the difference was statistically significant, and DBIL was significantly higher than that of serum. Therefore, EDTA-K2 anticoagulant plasma can be used for the detection of TBIL, TP, ALB, ALT and AST, but not for the detection of DBIL and ALP. The reference range of EDTA-K2 plasma should be formulated or multiplied by the correction coefficient for GGT detection results.

Application of dipotassium ethylenediaminetetraacetate (EDTA-2K):

1. Additive for vacuum blood collection vessel: the additive for vacuum blood collection vessel is potassium ethylenediamine tetraacetate (EDTA-2K) aqueous solution. 4mg of potassium ethylenediamine tetraacetate (EDTA-2K) is required for anticoagulation of 2ml blood. Because the concentration is small, in order not to dilute the blood, 20 g / L aqueous solution containing dipotassium ethylenediaminetetraacetate (EDTA-2K) is generally preset in each collecting vessel μ l. Since the validity period of the blood collection vessel is two years, when the service environment changes slightly, such as temperature change, the water is very easy to evaporate to the tube wall (especially the water in the solvent of plastic pet tube can penetrate through the tube wall and leak out), resulting in the rapid crystallization of EDTA ? 2K in the tube. The crystallized potassium ethylenediamine tetraacetate blood collection vessel is required to be upside down for at least 8 times during blood collection, Only in this way can the crystalline EDTA-2K be fully dissolved and mixed in the blood. If the action is slightly larger, the red blood cells will be destroyed and hemolyzed, and the platelets will gather, adhere and break.

2. Prepare toilet deodorant. By mass, it includes the following substances: 5 ~ 10 parts of acid salt, an appropriate amount of ammonia, 50 ~ 70 parts of alcohol, 5 ~ 10 parts of urotropine, an appropriate amount of dipotassium ethylenediamine tetraacetate, an appropriate amount of isobutanolamine and 60 ~ 80 parts of water. The deodorant can deodorize and prevent scale, has the advantages of simple use method, low dosage, long duration and no corrosion to the urinal. The production method is simple, the raw material cost is low, non-toxic, harmless and pollution-free.

3. Used as complexing agent in liquid phase analysis. If the invention provides a tetrabutylammonium bisulfate buffer salt system for liquid chromatography detection, the solvent is water, the solute includes tetrabutylammonium bisulfate and buffer ion pair, the buffer ion pair is composed of potassium dihydrogen phosphate and phosphoric acid, and the solute also includes a complexing agent, preferably potassium ethylenediamine tetraacetate. The tetrabutyl ammonium bisulfate buffer salt system provided above is stable within 24 hours without turbidity; Taking the analysis of related substances of moxifloxacin hydrochloride as an example, it can be seen that the chromatographic behavior of moxifloxacin, N-methyl impurity, impurity a, impurity B, impurity C, impurity D and impurity E on the reverse phase chromatographic column is basically the same between the tetrabutylammonium bisulfate buffer salt system placed at room temperature for 24 hours and the newly configured tetrabutylammonium bisulfate buffer salt system.

4. Prepare metal polishing agent. The polishing agent takes potassium ethylenediamine tetraacetate (EDTA-2K), trisodium phosphate, dibutyl phthalate, sodium silicate, inorganic acid, dicarboxylic acid compound, tetrameric ricinoleic acid, biological buffer, stabilizer, water-soluble surfactant and water as raw materials, and through scientific content ratio, the prepared polishing agent can effectively remove impurities on the metal surface, It is not only convenient to operate, but also has good polishing effect, which greatly improves the production efficiency, ensures the cleanliness and gloss of the metal surface, and improves the quality of the metal.

Desheng has been committed to the research and development of blood collection additives and has 15 years of production experience. It can provide customers with heparin lithium, heparin sodium, EDTA dipotassium and EDTA dipotassium. If you have any demand, please call for details.