Effect of reducing agent in Tris buffer

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1 Kilogram	Negotiable

1 Kilogram

Negotiable

Min.Order :1 Kilogram
Purity: ≥99%
Payment Terms : L/C,D/A,D/P,T/T,Other

Keywords

4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid

Quick Details

Appearance:White crystal powder
Application:4-hydroxyethyl piperazine ethylsulfonic acid is a biological buffer; reaction buffer, pre-hybridization buffer and hybridization buffer for isolation and analysis of RNA nuclear components; 3'-termina
PackAge:500g/bottle, 25kg/barrel (can be packed according to customer's requirements)
ProductionCapacity:500|Kilogram|Month
Storage:Room temperature storage
Transportation:Export for many years, qualified for shipping and air transportation

Superiority:

1. Factory production, more favorable prices, and regular products are available in stock;

2. Desheng company is an enterprise producing biological buffer products, providing you with more comprehensive services with a variety of products;

3. Desheng has been in this industry for 16 years. Our product knowledge and production experience are very rich

4. Desheng has been engaged in foreign trade since 2010. We have our own right to export.

Details:

Tris buffer has good compatibility with nucleic acid and protein, and is widely used in various biological experiments. Tris has many buffer solution systems, such as Tris hydrochloric acid, Tris acetic acid, TAE, tbe, etc. Many buffers also add reducing agents, such as DTT and TCEP.

Reducing agent was added to Tris buffer to protect the stability of protein. It is usually used in protein separation and purification, Western blot, protein identification and other experiments. Proteins are formed by dehydration and condensation of a variety of amino acids. Some may contain cysteine acid residues, which are easy to be oxidized and may lead to protein aggregation and precipitation. Therefore, adding reducing agents DTT and TCEP to Tris buffer can prevent the above problems.

Tris buffer is usually prepared at room temperature, while the reducing agent DTT is degraded at room temperature. If a reducing agent is added to the buffer, it needs to be frozen at low temperature or added to Tris when in use. Desheng has rich production and R & D experience in biological buffer and can provide Tris and other reagents.

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