What are the uses of HEPES buffer

Details:

HEPES   is a buffer for Biochemistry and molecular biology, which is often used in biological and biochemical research. HEPES is also an zwitterionic organic buffer, which is mostly used to stabilize the pH value of cell culture medium. HEPES buffer lacks the ability to complex with most metal ions, so it is recommended to be used as a non coordination buffer in metal ion solution. HEPES buffer is also one of good buffers. Although HEPES buffer is a very popular buffer in laboratories all over the world, what is the real purpose of HPES

Recently, I learned some knowledge about HEPES buffer from professional literature, so I want to share it with you in this paper, hoping to play a role in your future experiments and research.

Basic information of HEPES buffer:

1. CAS No.: 7365-45-9

2. Molecular weight: 238.3

3. Formula: c8h18n2o4s

4. Useful pH range: 6.8-8.2

5、 pKa（25℃）：7.45-7.65

Use of HEPES buffer:

1. Used in the study of most metal ions

2. It can replace Tris and phosphate in the study of metal ions

3. For environmental and biological research

4. As buffer and eluent in cation exchange chromatography

5. As a buffer in plant research

6. As buffer in gel electrophoresis

7. As a buffer for electroporation

8. For mammalian cell culture

9. As a buffer for in vitro fertilization and embryo culture

10. Used for dioctylcolic acid (BCA) analysis

11. It is used in the study of some cartilaginous amphibians

Precautions for use of HEPES buffer:

1. HEPES can affect the membrane potential of neurons

2. HEPES interferes with the determination of Lowry protein

3. HEPES is not suitable for redox studies

4. HEPES is toxic to small crustaceans

5. HEPES interferes with the phenol oxidation of peroxidase

6. HEPES will affect the self oxidation rate of iron

7. HEPES is not recommended for protein determination with Folin reagent

Nowadays, HEPES buffer is often used in many fields, so the market demand for HEPES buffer is very large. Therefore, the buffer merchants in the market are mixed, making the procurement very difficult. Here we recommend a manufacturer: Hubei xindesheng Material Co., Ltd.

Desheng company has been established for decades and has rich experience in production process and product R & D technology. As a professional manufacturer of biological buffers, Desheng also provides a series of high-quality biological buffers such as HEPES, Tris and mops. In addition, Desheng company can also provide special testing services and after-sales services according to customers' applications. In any case, Desheng must be your best choice to buy biological buffer. If you are interested in the company's products, you can enter the company's official website to contact customer service for details.