Application characteristics of trimethylol aminomethane buffer

Details:

Trimethylolaminomethane buffer, i.e. Tris buffer, is usually mixed with hydrochloric acid to adjust the pH value to prepare buffer, and some are adjusted with acetic acid, boric acid, glycine and other acids, while many other kinds of buffers such as bicine, mops, HEPES and caps are adjusted with sodium hydroxide alkali solution, which is the unique feature of Tris buffer.

Application of Tris in biochemical experiment

Basic properties of trimethylolaminomethane

At room temperature (25 ℃), the pKa of Tris is 8.1; according to the buffer theory, the effective buffer range of Tris buffer is between pH 7.0 and 9.2. The pH of Tris aqueous solution is about 10.5. Generally, hydrochloric acid is added to adjust the pH value to the required value to obtain the buffer of this pH value. The pKa of Tris is greatly affected by temperature, which will lead to the change of pH value. Attention should be paid to the influence of temperature.

Characteristics of Tris buffer

Trimethylolaminomethane itself is a weak base, and the aqueous solution is alkaline. Therefore, hydrochloric acid is used to adjust the buffer rather than sodium hydroxide. Tris molecular structure contains an amino group, which can condense with aldehyde. Therefore, the use of Tris buffer may be affected in the system containing aldehyde.

Tris buffer is suitable for nucleic acid related experiments

Tris buffer is usually neutral and weak alkaline. DNA nucleic acid molecules will be deprotonated in such a solution to improve the solubility. Therefore, Tris can be used as a good solvent for nucleic acid (also suitable for protein) , in nucleic acid related experiments, Tris is preferred as buffer. EDTA is often added to Tris hydrochloride buffer to make te buffer, which can inhibit nuclease, inhibit nucleic acid degradation and improve nucleic acid stability. Acetic acid or boric acid is often used to replace hydrochloric acid in electrophoresis buffer, such as tae buffer and tbe buffer used in nucleic acid electrophoresis experiments.

In addition to nucleic acid related experiments, Tris is often used in protein related experiments, such as protein growth experiments under different pH conditions. Tris is used as a buffer. Because Tris buffer does not contain metal ions and has low ionic strength, it can also be used for the formation of intermediate fibers of nematode lamin. Desheng is a buffer manufacturer and can provide Tris supply of more than tons.