Biological buffer HEPES vs PBS

Details:

HEPES  

Biological buffer is a chemical substance that can stabilize the pH value in the required range. It has many kinds and different pH value ranges. Therefore, it is widely used in major experiments. The addition of buffer can change the acidity and alkalinity in the experiment, which plays a vital role in the success of the experiment. In fact, biological buffers were not widely used before 1996. The pH value of traditional buffers cannot effectively buffer higher than ph7.5, so they are limited to many applications.

In 1996, Dr. Norman good developed zwitterionic buffers, which can effectively solve some problems encountered in traditional buffers, because some columns of biological buffers developed this time are close to our physiological pH value, and are non-toxic to cells and will not be absorbed through cell membrane. HEPES and PBS are two commonly used buffers.

Comparison between HEPES buffer and PBS buffer:

1. Effective pH range

HEPES：6.8-8.2 PBS：5.8-8.0

2. Effect on cells

HEPES: non toxic to cells. When using HEPES buffer system, you can see the maximum cell density and vitality.

PBS: non toxic to cells. Diluted cell culture samples in PBS may inadvertently reduce cell viability.

3. Relationship with metal ions

HEPES: does not produce complexes or precipitates with metal ions.

PBS: it will produce complexes or precipitates with some metal ions.

4. Changes under freezing conditions

HEPES: the pH value will not change when the solution is frozen.

PBS: the pH value will change obviously under the condition of solution freezing.

Based on the above points, it is not difficult to see that HEPES buffer has certain advantages over PBS buffer. HEPES is also the best choice of cell culture buffer. Desheng biochemical is a manufacturer specializing in the production of HEPES. Since its establishment in 2005, it has been committed to the research of biological buffer, only to produce high-quality products, if necessary, Welcome to our official website for consultation at any time! Looking forward to your letter.