What are the application advantages of HEPES buffer

Details:

Hydroxyethyl piperazine ethanesulfonic acid HEPES is a weak acid. As an zwitterionic buffer in biochemical detection, it has the advantage of no cytotoxicity compared with other buffers. Therefore, it is widely used in the culture medium or preservation solution of various cells and viruses. Although it is not as widely used as Tris, it has significant advantages in specific cases.

Biological buffer HEPES

1. Advantages of HEPES in production:

HEPES production process is pollution-free and waste free, which can realize cleaner production and has a good scientific and technological demonstration role; Moreover, it can be refined by multi-step chemical unit operation through extraction, crystallization, coupling separation, etc. with low-cost chemical raw materials under the action of high temperature, certain pressure and catalyst. It can be synthesized from hydroxyethyl piperazine and sodium chloroethyl sulfonate, or from sodium hydroxyethyl sulfonate and ethyl piperazine. Originally, its production technology used organic solvent (from solid phase to liquid phase wet process) for trial production. The wet process has large solvent consumption, high evaporation energy consumption and easy solvent residue. Now it adopts catalytic reverse crystallization method, which has significantly upgraded its technology and improved product quality.

2. HEPES is used as a buffer of enzyme at low temperature

Generally, the use condition of biological buffer is room temperature of 25 ℃ or higher (according to the optimal pH range of enzyme), but sometimes it needs to be tested at low temperature. Some buffers are obviously affected by low temperature and are not suitable for use. The decomposition ability of HEPES can be enhanced with the increase of temperature and weakened with the decrease of temperature. However, compared with other buffers, its decomposition constant changes little with temperature, which makes HEPES buffer a buffer that can better maintain the structure and function of enzyme at low temperature.

3. HEPES has no toxic and side effects on cells

HEPES buffer can control the constant pH range for a long time. Low concentration HEPES has no toxic effect on cells. It is often used in cell culture. For example, HEPES can be added to the culture solution or virus preservation solution of various bacteria and fungi to maintain the pH environment required by cells, bacteria and viruses.

In addition to being used in cell culture medium and virus preservation solution, HEPES is also used in many in vitro diagnostic kits containing enzymes and even cosmetics containing buffers. Desheng is a manufacturer of biological buffers, which can provide HEPES, Tris, caps and other biological buffers in sufficient quantity.