How luminol performs luminescence detection

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Luminol , also known as luminescent ammonia, is fully known as 3-aminobenzoyl hydrazine. Luminol is widely used as a chemiluminescence reagent and is often used in chemiluminescence immunoassay biochemical system. Yellow crystal or beige powder at room temperature. It is a relatively stable chemical reagent.

Adding catalyst is a common method at present. Generally speaking, the chemiluminescence reaction between hydrogen peroxide and luminol luminescence system is particularly slow, but the reaction becomes particularly rapid after adding some catalysts.

At present, the main catalysts of luminol include some metal complexes and transition metal ions. Metal complexes mainly include hemoglobin and peroxidase; Transition metal ions mainly include Fe3 +, Fe2 +, Mn2 +, CR2 +, etc. Peroxidase, especially horseradish peroxidase, is commonly used in chemiluminescence immunoassay. Some compounds can react with peroxidase labeled antibodies, and then these compounds or antibodies are determined by luminol chemiluminescence. For example, digoxin and hepatitis B surface antigen are detected and analyzed by this method.

In addition to the accelerating effect of catalyst on detection, some inhibitors were also found in the study. These organics can inhibit the chemiluminescence of luminol, such as reducing compounds containing phenolic hydroxyl groups. During the reaction, they can react with oxidants to reduce the oxidant concentration and chemiluminescence intensity, so as to indirectly determine such organics.

Another method is indirect determination by coupling reaction. Here, coupling reaction refers to the combination of a reaction that can produce or consume chemiluminescence reactants and another chemiluminescence reaction, so as to realize the Indirect Chemiluminescence Determination of some substances. For example, some substrates produce hydrogen peroxide under the action of some enzymes, and then produce chemiluminescence with luminol. By measuring chemiluminescence, the purity of the measured substrate can be known indirectly.

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