Frequently asked questions about Tris HCl

Details:

What is the difference between Tris HCl powder and solution reagent

Tris HCl powder reagent and solution reagent are not only the difference between powder and solution, but also the use of powder reagent dissolved in water to form solution is different from that of finished solution reagent. The reason is that the molecular structure of trimethylolaminomethane (hoch2) 3cnh2 contains an amino group, which reacts 1:1 with the salt formed by hydrochloric acid, but there may be a small amount of Tris base in the powder product that does not react completely with hydrochloric acid.

What is the relationship between Tris HCl and Tris CL

These two are the same reagent, but the writing is different. Tris HCl is to add an appropriate amount of hydrochloric acid to Tris base solution to adjust it to the pH value you need. Tris series buffer (or HEPES) can be treated with DEPC, but this kind of amine containing reagent will accelerate the hydrolysis of DEPC. However, if you must treat with DEPC, increasing the final concentration of DEPC to 1% will achieve the same effect as 0.1%.

Does the pH value of Tris HCl buffer change with temperature

The pH of Tris HCl buffer is greatly affected by temperature. Once the temperature changes, the pH will change significantly. The pH at 37 ℃ is 0.18 lower than that at 23 ℃. The average pH is 0.32 lower than that at 12 ℃, and the temperature change has a great impact on the pH value of the buffer. Therefore, it must be prepared at the service temperature. The tris HCl buffer prepared at room temperature cannot be used at 0 ℃ ~ 4 ℃.

What is the difference between Tris and Tris HCl

Tris is an organic compound, which is mainly used in molecular biology to make buffer or as basic buffer. Tris HCl is a buffer commonly used by molecular biologists to adjust or stabilize the pH value of the solution. Whether to use Tris HCl or Tris depends on the ongoing experiment. When the pH value of the solution needs to be adjusted, conjugated acidic or alkaline components can be used. For example, if there is a scheme requiring Tris HCl, Tris can be used to adjust pH. (Tris and Tris HCl are the same when they are prepared into buffer)

Differences and uses of Tris HCl and PBS buffer

The pKa value of PBS solution will change with the dilution of buffer, and inhibit the activities of most enzymes, including kinase, phosphorylase, dehydrogenase, etc. It is pH7 4 phosphate buffer, pH is fixed and isotonic with human blood, so it is generally used in molecular cloning and cell culture experiments.

Tris HCl is prepared with trimethylolaminomethane and hydrochloric acid, and then the pH can be adjusted. All pH segments are useful. Ph6 is often used in protein experiments 8 and pH8 8. Generally, this is also used in protein, DNA and RNA experiments in laboratories.